{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,26]],"date-time":"2026-02-26T15:07:22Z","timestamp":1772118442090,"version":"3.50.1"},"reference-count":49,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2003,1,14]],"date-time":"2003-01-14T00:00:00Z","timestamp":1042502400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cell Motil. Cytoskeleton"],"published-print":{"date-parts":[[2003,2]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Neutrophils migrate rapidly by co\u2010ordinating regulation of their \u03b22\u2010integrin adhesion with turnover of filamentous F\u2010actin. The seven\u2010protein Arp2\/3 complex regulates actin polymerisation upon activation by proteins of the WASP\u2010family. To investigate links between actin polymerisation, adhesion, and migration, we used a novel osmotic\u2010shock method to load neutrophils with peptides: (1) WASP\u2010WA and Scar\u2010WA (which incorporate the actin\u2010 and Arp2\/3\u2010binding regions of WASP and Scar1), to compete with endogenous WASP\u2010family members; (2) proline rich motifs (PRM) from the ActA protein of\n                    <jats:italic>L. monocytogenes<\/jats:italic>\n                    or from vinculin, which bind vasodilator\u2010stimulated phosphoprotein (VASP), a regulator of cytoskeleton assembly. In a flow system, rolling\u2010adherent neutrophils were stimulated with formyl tri\u2010peptide. This caused rapid immobilisation, followed by migration with increasing velocity, supported by activated \u03b22\u2010integrin CD11b\/CD18. Loading ActA PRM (but not vinculin PRM) caused concentration\u2010dependent reduction in migration velocity. At the highest concentration, unstimulated neutrophils had elevated F\u2010actin and were rigid, but could not change their F\u2010actin content or shape upon stimulation. Scar\u2010WA also caused marked reduction in migration rate, but WASP\u2010WA had a lesser effect. Scar\u2010WA did not modify activation\u2010dependent formation of F\u2010actin or change in shape. However, a reduction in rate of downregulation of integrin adhesion appeared to contribute to impaired migration. These studies show that interference in cytoskeletal reorganisation that follows activation in neutrophils, can impair regulation of integrin function as well as motility. They also suggest a role of the Arp2\/3 complex and WASP\u2010family in co\u2010ordinating actin polymerisation and integrin function in migrating neutrophils. Cell Motil. Cytoskeleton 54:135\u2013146, 2003. \u00a9 2003 Wiley\u2010Liss, Inc.\n                  <\/jats:p>","DOI":"10.1002\/cm.10091","type":"journal-article","created":{"date-parts":[[2003,1,16]],"date-time":"2003-01-16T16:26:15Z","timestamp":1042734375000},"page":"135-146","source":"Crossref","is-referenced-by-count":22,"title":["Linked regulation of motility and integrin function in activated migrating neutrophils revealed by interference in remodelling of the cytoskeleton"],"prefix":"10.1002","volume":"54","author":[{"given":"Stephen I.","family":"Anderson","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Barbara","family":"Behrendt","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Laura M.","family":"Machesky","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robert 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