{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T00:33:54Z","timestamp":1792024434023,"version":"4.4.0"},"reference-count":24,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2005,11,29]],"date-time":"2005-11-29T00:00:00Z","timestamp":1133222400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cell Motil. Cytoskeleton"],"published-print":{"date-parts":[[2006,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The WAVE\/Scar proteins regulate actin polymerisation at the leading edge of motile cells via activation of the Arp2\/3 complex in response to extracellular cues. Within cells they form part of a pentameric complex that is thought to regulate their ability to interact and activate the Arp2\/3 complex. However, the exact mechanism for this is not known. We set out to assess whether phosphorylation of Scar1 by the non\u2010receptor tyrosine kinase Src may influence the function of Scar1 and its ability to regulate Arp2\/3\u2010mediated actin polymerisation. We show that Scar1 is phosphorylated by Src in vitro and in vivo and identify tyrosine 125 as the major site in Scar1 to be phosphorylated in cells. Src\u2010dependent phosphorylation of Scar1 on tyrosine 125 enhances its ability to bind to the Arp2\/3 complex and regulates its ability to control actin polymerisation in cells. Thus, Src may act as an intermediary to regulate the activity of the Arp2\/3 complex in response to external stimuli, via modulation of its interaction with WAVE\/Scar proteins. Cell Motil. Cytoskeleton, 2006. \u00a9 2005 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/cm.20101","type":"journal-article","created":{"date-parts":[[2005,11,29]],"date-time":"2005-11-29T19:23:43Z","timestamp":1133292223000},"page":"6-13","source":"Crossref","is-referenced-by-count":43,"title":["Src\u2010dependent phosphorylation of Scar1 promotes its association with the Arp2\/3 complex"],"prefix":"10.1002","volume":"63","author":[{"given":"Hazel","family":"Ardern","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emma","family":"Sandilands","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura M.","family":"Machesky","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul","family":"Timpson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Margaret C.","family":"Frame","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Valerie G.","family":"Brunton","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2005,11,29]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200403127"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-04-1949"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M203346200"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1097-2765(03)00172-2"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature00859"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrm779"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1097-2765(01)00393-8"},{"issue":"9","key":"e_1_2_1_9_1","doi-asserted-by":"crossref","first-page":"1699","DOI":"10.1242\/jcs.114.9.1699","article-title":"Phosphorylation of p190 on Tyr1105 by c\u2010Src is necessary but not sufficient for EGF\u2010induced actin disassembly in C3H10T1\/2 fibroblasts","volume":"114","author":"Haskell MD","year":"2001","journal-title":"J Cell Sci"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncb1105"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/35004513"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0409120102"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0960-9822(98)00015-3"},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.96.7.3739"},{"key":"e_1_2_1_15_1","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/17.23.6932"},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1101509"},{"key":"e_1_2_1_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/nsb952"},{"key":"e_1_2_1_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(03)00120-X"},{"key":"e_1_2_1_19_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.131.3.761"},{"key":"e_1_2_1_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2004.09.019"},{"key":"e_1_2_1_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tcb.2004.04.007"},{"key":"e_1_2_1_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1534-5807(02)00324-6"},{"key":"e_1_2_1_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1534-5807(03)00297-1"},{"key":"e_1_2_1_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0960-9822(01)00583-8"},{"key":"e_1_2_1_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1097-2765(03)00139-4"}],"container-title":["Cell Motility"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fcm.20101","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cm.20101","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,15]],"date-time":"2023-11-15T17:19:40Z","timestamp":1700068780000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/cm.20101"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2005,11,29]]},"references-count":24,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2006,1]]}},"alternative-id":["10.1002\/cm.20101"],"URL":"https:\/\/doi.org\/10.1002\/cm.20101","archive":["Portico"],"relation":{},"ISSN":["0886-1544"],"issn-type":[{"value":"0886-1544","type":"print"}],"subject":[],"published":{"date-parts":[[2005,11,29]]}}}