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We show that all three isoforms PFN\u20101, PFN\u20102, and PFN\u20103 are expressed in vivo and biochemical studies indicate they bind actin and influence actin dynamics in a similar manner. In addition, they bind poly(<jats:sc>L<\/jats:sc>\u2010proline) and phosphatidylinositol 4,5\u2010bisphosphate micelles. PFN\u20101 is essential whereas PFN\u20102 and PFN\u20103 are nonessential. Immunostainings revealed different expression patterns for the profilin isoforms. In embryos, PFN\u20101 localizes in the cytoplasm and to the cell\u2013cell contacts at the early stages, and in the nerve ring during later stages. During late embryogenesis, expression of PFN\u20103 was specifically detected in body wall muscle cells. In adult worms, PFN\u20101 is expressed in the neurons, the vulva, and the somatic gonad, PFN\u20102 in the intestinal wall, the spermatheca, and the pharynx, and PFN\u20103 localizes in a striking dot\u2010like fashion in body wall muscle. Thus the model organism <jats:italic>Caenorhabditis elegans<\/jats:italic> expresses three profilin isoforms and is the first invertebrate animal with tissue\u2010specific profilin expression. Cell Motil. Cytoskeleton, 2006.\u00a9 2005 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/cm.20102","type":"journal-article","created":{"date-parts":[[2005,11,29]],"date-time":"2005-11-29T19:23:47Z","timestamp":1133292227000},"page":"14-28","source":"Crossref","is-referenced-by-count":40,"title":["<i>Caenorhabditis elegans<\/i> expresses three functional profilins in a tissue\u2010specific manner"],"prefix":"10.1002","volume":"63","author":[{"given":"D.","family":"Polet","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Lambrechts","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K.","family":"Ono","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Mah","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F.","family":"Peelman","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Vandekerckhove","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D. 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