{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T20:36:10Z","timestamp":1791059770395,"version":"4.1.0"},"reference-count":24,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2008,11,14]],"date-time":"2008-11-14T00:00:00Z","timestamp":1226620800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cell Motil. Cytoskeleton"],"published-print":{"date-parts":[[2009,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The (Wiskott\u2010Aldrich Syndrome Protein)\u2010family verprolin homologous protein (WAVE) family of proteins occupies a pivotal position in the cell, converting extracellular signals into the formation of branched filamentous (F) actin structures. WAVE proteins contain a verprolin central acidic (VCA) domain at their C\u2010terminus, responsible for binding to and activating the Arp2\/3 complex, which in\u2010turn nucleates the formation of new actin filaments. Here we identify five Casein Kinase 2 (CK2) phosphorylation sites within the VCA domain of WAVE2, serines 482, 484, 488, 489, and 497. Phosphorylation of these sites is required for a high affinity interaction with the Arp2\/3 complex. Phosphorylation of ser 482 and 484 specifically inhibits the activation of the Arp2\/3 complex by the WAVE2 VCA domain, but has no effect on the affinity for the Arp2\/3 complex when the other phosphorylation sites are occupied. We demonstrate phosphorylation of all five sites on endogenous WAVE2 and show that their mutation to non\u2010phosphorylatable alanine residues inhibits WAVE2 function in vivo, inhibiting cell ruffling and disrupting the integrity of the leading edge of migrating cells. Cell Motil. Cytoskeleton 2008. \u00a9 2008 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/cm.20323","type":"journal-article","created":{"date-parts":[[2008,11,14]],"date-time":"2008-11-14T15:42:15Z","timestamp":1226677335000},"page":"36-47","source":"Crossref","is-referenced-by-count":47,"title":["WAVE2 is regulated by multiple phosphorylation events within its VCA domain"],"prefix":"10.1002","volume":"66","author":[{"given":"Shirin M.","family":"Pocha","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giles O.","family":"Cory","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2008,11,14]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/cm.20101"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M705894200"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1097-2765(03)00172-2"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.013714"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2004.09.018"},{"issue":"6","key":"e_1_2_1_7_1","doi-asserted-by":"crossref","first-page":"1243","DOI":"10.1042\/BST0331243","article-title":"Regulation of actin assembly by SCAR\/WAVE proteins","volume":"33","author":"Ibarra N","year":"2005","journal-title":"Biochem Soc Trans"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M507470200"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature04976"},{"issue":"19","key":"e_1_2_1_10_1","doi-asserted-by":"crossref","first-page":"9136","DOI":"10.1016\/S0021-9258(18)48058-1","article-title":"Substrate specificity determinants for casein kinase II as deduced from studies with synthetic peptides","volume":"262","author":"Kuenzel EA","year":"1987","journal-title":"J Biol Chem"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2003.10.005"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0409120102"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.96.7.3739"},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.E02-06-0345"},{"key":"e_1_2_1_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/35050590"},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.02-0473rev"},{"key":"e_1_2_1_17_1","doi-asserted-by":"publisher","DOI":"10.1093\/jb\/mvm034"},{"key":"e_1_2_1_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/nsb952"},{"key":"e_1_2_1_19_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.biophys.35.040405.101936"},{"key":"e_1_2_1_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tcb.2006.08.006"},{"key":"e_1_2_1_21_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1066333"},{"key":"e_1_2_1_22_1","doi-asserted-by":"publisher","DOI":"10.1038\/nsmb870"},{"key":"e_1_2_1_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbamcr.2006.10.005"},{"key":"e_1_2_1_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1534-5807(03)00297-1"},{"key":"e_1_2_1_25_1","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/cdg350"}],"container-title":["Cell Motility"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fcm.20323","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cm.20323","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,14]],"date-time":"2023-11-14T12:35:05Z","timestamp":1699965305000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/cm.20323"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2008,11,14]]},"references-count":24,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2009,1]]}},"alternative-id":["10.1002\/cm.20323"],"URL":"https:\/\/doi.org\/10.1002\/cm.20323","archive":["Portico"],"relation":{},"ISSN":["0886-1544"],"issn-type":[{"value":"0886-1544","type":"print"}],"subject":[],"published":{"date-parts":[[2008,11,14]]}}}