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Immediately after a wild\u2010type cell suspension was vortexed, the average swimming velocity of cells increased from 130 \u03bcm\/second to 150 \u03bcm\/second, due to an elevation of flagellar beat frequency from \u223c60 Hz to \u223c70 Hz without detectable change in the flagellar waveforms. This response required outer arm dynein. Treatment with EGTA, Ca<jats:sup>2+<\/jats:sup>\u2010channel blockers, or mechanosensitive\u2010channel blockers inhibited it. In demembranated and reactivated cell models, a modest increase in Ca<jats:sup>2+<\/jats:sup> concentration elevated the axonemal beat frequency. These data indicate that the mechanical agitation increases beat frequency because it causes Ca<jats:sup>2+<\/jats:sup> influx into flagella, which then activates outer arm dynein. Cell Motil. 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