{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T01:57:53Z","timestamp":1791338273139,"version":"4.3.1"},"reference-count":55,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2010,2,6]],"date-time":"2010-02-06T00:00:00Z","timestamp":1265414400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cytoskeleton"],"published-print":{"date-parts":[[2010,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Activators of the Arp2\/3 complex, termed nucleation\u2010promoting factors (NPFs), are required for the proper spatial and temporal control of actin assembly in cells. Mammalian cells express several NPFs, each of which functions in a distinct cellular process, including WASP and N\u2010WASP in phagocytosis and endocytosis, WAVE and JMY in cell migration, and WHAMM in ER\u2010to\u2010Golgi transport. Although another NPF called WASH was recently identified, the cellular localization and function of this protein were unclear. Here we demonstrated that human WASH alone potently activated the Arp2\/3 complex in vitro and in cells, suggesting that the protein is not autoinhibited like N\u2010WASP, but is likely regulated by interacting proteins. In cells, WASH was associated with Rab5\u2010positive early endosomes and Rab11\u2010positive recycling endosomes that were enriched for actin filaments. Silencing of WASH or Arp2\/3 complex expression by RNAi, or disruption of actin function by drug treatments, caused enlargement and elongation of endosomes. Intriguingly, WASH silencing, as well as actin disruption, delayed EGF transport to LAMP1\u2010positive late endosomes. These observations indicate that actin polymerization by WASH influences the shape and maturation of endosomes, and highlight a previously unrecognized role for WASH and the Arp2\/3 complex in the degradative steps of endocytic trafficking. \u00a9 2010 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/cm.20437","type":"journal-article","created":{"date-parts":[[2010,2,6]],"date-time":"2010-02-06T17:21:10Z","timestamp":1265476870000},"page":"193-206","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":207,"title":["WASH and the Arp2\/3 complex regulate endosome shape and trafficking"],"prefix":"10.1002","volume":"67","author":[{"given":"Steve N.","family":"Duleh","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthew D.","family":"Welch","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2010,2,19]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2007.11.011"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M204145200"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.02444"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2818.2006.01706.x"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2008.05.032"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M010387200"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0960-9822(03)00131-3"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/cm.20342"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2009.09.010"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature00859"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.cellbio.19.111401.093127"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2004.09.018"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrm2026"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2009.09.009"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/35096054"},{"key":"e_1_2_7_17_1","doi-asserted-by":"crossref","first-page":"84","DOI":"10.1111\/j.1462-5822.2009.01380.x","article-title":"Molecular dissection of Salmonella\u2010induced membrane ruffling versus invasion","volume":"12","author":"Hanisch J","year":"2009","journal-title":"Cell Microbiol"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.8.3.533"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncb1304"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1038\/nsmb.1587"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrm1940"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1038\/35004513"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.0030237"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.035246"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0960-9822(98)00015-3"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1038\/35050590"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrm1315"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/cdg321"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1078\/0171-9335-00356"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1038\/34208"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1038\/35047107"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2009.01.007"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-05-2916"},{"key":"e_1_2_7_34_1","first-page":"18","article-title":"Endocytosis assays in intact and permeabilized cells","volume":"11","author":"Osborne A","year":"2005","journal-title":"Curr Protoc Cell Biol"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2005.10.002"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ceb.2005.06.008"},{"key":"e_1_2_7_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(03)00120-X"},{"key":"e_1_2_7_38_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.290.5492.801"},{"key":"e_1_2_7_39_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00018-009-0001-y"},{"key":"e_1_2_7_40_1","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.e04-08-0734"},{"key":"e_1_2_7_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)80732-1"},{"key":"e_1_2_7_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0960-9822(00)00384-5"},{"key":"e_1_2_7_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2008.06.012"},{"key":"e_1_2_7_44_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0409817102"},{"key":"e_1_2_7_45_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncb1001-897"},{"key":"e_1_2_7_46_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrm2060"},{"key":"e_1_2_7_47_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrm2728"},{"key":"e_1_2_7_48_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tcb.2004.04.007"},{"key":"e_1_2_7_49_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ceb.2005.12.003"},{"key":"e_1_2_7_50_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200509067"},{"key":"e_1_2_7_51_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrm2069"},{"key":"e_1_2_7_52_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.148.3.519"},{"key":"e_1_2_7_53_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.138.2.375"},{"key":"e_1_2_7_54_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.cellbio.18.040202.112133"},{"key":"e_1_2_7_55_1","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.e04-09-0774"},{"key":"e_1_2_7_56_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncb1852"}],"container-title":["Cytoskeleton"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fcm.20437","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cm.20437","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,31]],"date-time":"2023-10-31T01:27:21Z","timestamp":1698715641000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/cm.20437"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,2,19]]},"references-count":55,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2010,3]]}},"alternative-id":["10.1002\/cm.20437"],"URL":"https:\/\/doi.org\/10.1002\/cm.20437","archive":["Portico"],"relation":{},"ISSN":["1949-3584","1949-3592"],"issn-type":[{"value":"1949-3584","type":"print"},{"value":"1949-3592","type":"electronic"}],"subject":[],"published":{"date-parts":[[2010,2,19]]},"assertion":[{"value":"2009-12-11","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2010-01-27","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2010-02-19","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}