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Although there is no difference prior to differentiation, a striking difference is seen after differentiation. Both \u03b2I and \u03b2III occur in cell bodies and neurites, while \u03b2II occurs mostly in neurites. Knocking down \u03b2I causes a large decrease in cell viability while silencing \u03b2II and \u03b2III does not. Knocking down \u03b2II causes a large decrease in neurite outgrowth without affecting viability. Knocking down \u03b2III has little effect on neurite outgrowth and only decreases viability if cells are treated with glutamate and glycine, a combination known to generate free radicals and reactive oxygen species. It appears, therefore, that \u03b2I is required for cell viability, \u03b2II for neurite outgrowth and \u03b2III for protection against free radicals and reactive oxygen species. \u00a9 2010 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/cm.20455","type":"journal-article","created":{"date-parts":[[2010,5,10]],"date-time":"2010-05-10T23:28:30Z","timestamp":1273534110000},"page":"431-441","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":83,"title":["The \u03b2 isotypes of tubulin in neuronal differentiation"],"prefix":"10.1002","volume":"67","author":[{"given":"Jiayan","family":"Guo","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Consuelo","family":"Walss\u2010Bass","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richard F.","family":"Ludue\u00f1a","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2010,6,18]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuroscience.2006.09.062"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.85.21.8335"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi00439a040"},{"key":"e_1_2_7_5_1","doi-asserted-by":"crossref","first-page":"3029","DOI":"10.1016\/S0021-9258(18)69171-9","article-title":"A monoclonal antibody against the type II isotype of \u03b2\u2010tubulin. 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