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Mature seminal vesicle sperm from the water strider <jats:italic>Aquarius remigis<\/jats:italic> are immotile in vitro, but vigorous motility is activated by trypsin. Trypsin\u2010activated motility was blocked by pretreatment of the sperm with BAPTA\u2010AM to chelate intracellular Ca<jats:sup>2+<\/jats:sup> and was partially rescued by subsequent addition of A23187 and Ca<jats:sup>2+<\/jats:sup>. Thapsigargin stimulated motility in the absence of trypsin, suggesting that intracellular Ca<jats:sup>2+<\/jats:sup> stores are available. In addition, motility could be fully activated by the phosphatase inhibitor calyculin A, suggesting that the immotile state is maintained by an endogenous phosphatase and that kinase activity is required for motility. The MEK1\/2 inhibitor U0126 significantly reduced trypsin activated motility, and MPM\u20102, an antibody which recognizes proline\u2010directed phosphorylation by kinases such as MAPK, recognized components of the water strider sperm flagellum. Antibodies specific for the mouse protease activated receptor PAR2 recognized an antigen on the sperm flagellum. These results suggest that trypsin stimulates a Ca<jats:sup>2+<\/jats:sup> and MAPK mediated signaling pathway and potentially implicate a PAR2\u2010like protein in regulating motility. \u00a9 2012 Wiley Periodicals, Inc.<\/jats:p>","DOI":"10.1002\/cm.21012","type":"journal-article","created":{"date-parts":[[2012,1,25]],"date-time":"2012-01-25T17:24:14Z","timestamp":1327512254000},"page":"207-220","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":30,"title":["Protease activation and the signal transduction pathway regulating motility in sperm from the water strider <i>Aquarius remigis<\/i>"],"prefix":"10.1002","volume":"69","author":[{"given":"Haruhiko","family":"Miyata","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Catherine D.","family":"Thaler","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Leah T.","family":"Haimo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Richard A.","family":"Cardullo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2012,2,14]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/0020-1790(87)90075-8"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/0965-1748(94)90134-1"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M710492200"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1017\/CBO9780511721946.009"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-ento-120709-144823"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0065-2806(08)60279-9"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.86.2.446"},{"key":"e_1_2_6_9_1","doi-asserted-by":"crossref","first-page":"8801","DOI":"10.1016\/S0021-9258(18)31518-7","article-title":"Identification of intracellular calcium pools. 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