{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T07:32:01Z","timestamp":1788939121090,"version":"build-2803163510"},"reference-count":38,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2012,2,21]],"date-time":"2012-02-21T00:00:00Z","timestamp":1329782400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cytoskeleton"],"published-print":{"date-parts":[[2012,4]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Primary cilia are organelles that extend from the cell surface. More than 600 proteins have been identified in cilia, but ciliary targeting mechanisms are poorly understood. Nephronophthisis (NPHP) is an autosomal recessive cystic kidney disease with 11 responsible genes (\n                    <jats:italic>NPHP1\u201311<\/jats:italic>\n                    ) thus far being identified. The mouse\n                    <jats:italic>Nphp3<\/jats:italic>\n                    gene product is localized in the cilia and contains coiled\u2010coil (CC) domains and tetratricopeptide repeats, but the ciliary targeting sequences (CTSs) are unknown. In the present study, we generated a series of GFP\u2010tagged deletion constructs of Nphp3 and tried to find the CTSs of Nphp3. We found that the N\u2010terminal 201 amino acid fragment (Nphp3 [1\u2013201]), which contains two CC domains, is necessary and sufficient for cilia localization. Further analysis revealed that an N\u2010terminal glycine (G2), which is a conserved myristoylation site among vertebrates, is also essential for trafficking of Nphp3 to the ciliary shaft. Interestingly, the N\u2010terminal fragments, Nphp3 (8\u2013201), Nphp3 (52\u2013201), and Nphp3 (96\u2013201), that contain the CC domains, targeted the basal body, but could not enter into the ciliary shaft. Our results showed the importance of myristoylation in ciliary trafficking, and suggest that Nphp3 trafficking to the ciliary shaft occurs in a two\u2010step process. \u00a9 2012 Wiley Periodicals, Inc.\n                  <\/jats:p>","DOI":"10.1002\/cm.21014","type":"journal-article","created":{"date-parts":[[2012,2,10]],"date-time":"2012-02-10T11:50:30Z","timestamp":1328874630000},"page":"221-234","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":42,"title":["Targeting of Nphp3 to the primary cilia is controlled by an N\u2010terminal myristoylation site and coiled\u2010coil 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