{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,8]],"date-time":"2025-10-08T15:52:42Z","timestamp":1759938762757},"reference-count":34,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2015,6,30]],"date-time":"2015-06-30T00:00:00Z","timestamp":1435622400000},"content-version":"vor","delay-in-days":29,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cytoskeleton"],"published-print":{"date-parts":[[2015,6]]},"abstract":"<jats:p>Cytoplasmic actin isoforms beta (\u03b2\u2010) and gamma (\u03b3\u2010) perform crucial physiological roles in inner ear hair cells (HC). The stereocilium, which is structured by parallel actin filaments composed of both isoforms, is the responsive organelle to mechanical stimuli such as sound, gravity and head movements. Modifications in isoform proportions affect the function of the stereocilia as previously shown in genetic studies of mutant mice. Here, immunogold labeling TEM studies in mice showed that both \u03b2\u2010 and \u03b3\u2010actin isoforms colocalize throughout stereocilia actin filaments, adherens junctions and cuticular plates as early as embryonic stage 16.5. Gold\u2010particle quantification indicated that there was 40% more \u03b3\u2010 actin than \u03b2\u2010actin at E16.5. In contrast, \u03b2\u2010 and \u03b3\u2010actin were equally concentrated in adult stereocilia of cochlear and vestibular HC. Interestingly, all actin\u2010based structures presented almost five\u2010fold more \u03b2\u2010actin than \u03b3\u2010actin in 22 month\u2010 old mice, suggesting that \u03b3\u2010actin is probably under\u2010expressed during the aging process. These data provide evidence of dynamic modifications of the actin isoforms in stereocilia, cuticular plates and cell junctions during the whole HC life. \u00a9 2015 Wiley Periodicals, Inc.<\/jats:p>","DOI":"10.1002\/cm.21227","type":"journal-article","created":{"date-parts":[[2015,5,30]],"date-time":"2015-05-30T05:14:00Z","timestamp":1432962840000},"page":"282-291","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Evidence for changes in beta\u2010 and gamma\u2010actin proportions during inner ear hair cell life"],"prefix":"10.1002","volume":"72","author":[{"given":"Leonardo R.","family":"Andrade","sequence":"first","affiliation":[{"name":"Laboratory of Cell Structure and Dynamics National Institute on Deafness and Other Communication Disorders, National Institutes of Health Bethesda, MD 20892 USA"},{"name":"Laboratory of Biomineralization, Institute of Biomedical Sciences, Federal University of Rio de Janeiro, 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