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Despite increasing knowledge on the interaction between ECTV and its natural host\u2014the mouse\u2014surprisingly, still little is known about the cell biology of ECTV infection. Because pathogen interaction with the cytoskeleton is still a growing area of research in the virus\u2013host cell interplay, the aim of the present study was to evaluate the consequences of ECTV infection on the cytoskeleton in a murine fibroblast cell line. The viral effect on the cytoskeleton was reflected by changes in migration of the cells and rearrangement of the architecture of tubulin, vimentin, and actin filaments. The virus\u2010induced cytoskeletal rearrangements observed in these studies contributed to the efficient cell\u2010to\u2010cell spread of infection, which is an important feature of ECTV virulence. Additionally, during later stages of infection L929 cells produced two main types of actin\u2010based cellular protrusions: short (actin tails and \u201cdendrites\u201d) and long (cytoplasmic corridors). Due to diversity of filopodial extensions induced by the virus, we suggest that ECTV represents a valuable new model for studying processes and pathways that regulate the formation of cytoskeleton\u2010based cellular structures. \u00a9 2016 Wiley Periodicals, Inc.<\/jats:p>","DOI":"10.1002\/cm.21308","type":"journal-article","created":{"date-parts":[[2016,5,12]],"date-time":"2016-05-12T09:40:38Z","timestamp":1463046038000},"page":"396-417","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Remodeling of the fibroblast cytoskeletal architecture during the replication cycle of Ectromelia virus: A morphological in vitro study in a murine cell line"],"prefix":"10.1002","volume":"73","author":[{"given":"Lidia","family":"Szulc\u2010Dabrowska","sequence":"first","affiliation":[{"name":"Division of Immunology, Department of Preclinical Sciences, Faculty of Veterinary Medicine Warsaw University of Life Sciences\u2010SGGW 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