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In order to function properly, stereocilia must attain precise sizes in different hair cell types and must coordinately form distinct rows with varying lengths. Espins are actin\u2010bundling proteins that have a well\u2010characterized role in stereocilia formation; loss of function mutations in Espin result in shorter stereocilia and deafness in the <jats:italic>jerker<\/jats:italic> mouse. Here we describe the generation of an Espin overexpressing transgenic mouse line that results in longer first row stereocilia and discoordination of second\u2010row stereocilia length. Furthermore, Espin overexpression results in the misregulation of other stereocilia factors including GNAI3, GPSM2, EPS8, WHRN, and MYO15A, revealing that GNAI3 and GPSM2 are dispensable for stereocilia overgrowth. Finally, using an in vitro actin polymerization assay we show that espin provides an anti\u2010capping function that requires both the G\u2010actin binding WH2 domain as well as either the C\u2010terminal F\u2010actin binding domain or the internal xAB actin\u2010binding domain. Our results provide a novel function for Espins at the barbed ends of actin filaments distinct from its previous known function of actin bundling that may account for their effects on stereocilia growth.<\/jats:p>","DOI":"10.1002\/cm.21719","type":"journal-article","created":{"date-parts":[[2022,7,18]],"date-time":"2022-07-18T07:30:19Z","timestamp":1658129419000},"page":"64-74","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Espin overexpression causes stereocilia defects and provides an anti\u2010capping effect on actin polymerization"],"prefix":"10.1002","volume":"79","author":[{"given":"Lili","family":"Zheng","sequence":"first","affiliation":[{"name":"Department of Cell and Developmental Biology Northwestern University, Feinberg School of Medicine  Chicago Illinois USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephen A.","family":"Adam","sequence":"additional","affiliation":[{"name":"Department of Cell and Developmental Biology Northwestern University, Feinberg School of Medicine  Chicago Illinois USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jaime","family":"Garc\u00eda\u2010Anoveros","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology Neurology and Neuroscience Northwestern University, Feinberg School of Medicine  Chicago Illinois USA"},{"name":"Hugh Knowles Center for Clinical and Basic Science in Hearing and its Disorders Northwestern University, Feinberg School of Medicine  Chicago Illinois USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brian J.","family":"Mitchell","sequence":"additional","affiliation":[{"name":"Department of Cell and Developmental Biology Northwestern University, Feinberg School of Medicine  Chicago Illinois USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James R.","family":"Bartles","sequence":"additional","affiliation":[{"name":"Department of Cell and Developmental Biology Northwestern University, Feinberg School of Medicine  Chicago Illinois USA"},{"name":"Hugh Knowles Center for Clinical and Basic Science in Hearing and its Disorders Northwestern University, Feinberg School of Medicine  Chicago Illinois USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,7,30]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1001\/archinternmed.2009.66"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/s0955-0674(99)00059-9"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.109.6.1229"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.143.1.107"},{"key":"e_1_2_9_6_1","first-page":"1","article-title":"Summary health statistics for U.S. adults: National health interview survey, 2012","volume":"10","author":"Blackwell D. 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