{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T14:38:08Z","timestamp":1786977488884,"version":"build-2736575974"},"reference-count":53,"publisher":"Wiley","issue":"7-8","license":[{"start":{"date-parts":[[2023,6,2]],"date-time":"2023-06-02T00:00:00Z","timestamp":1685664000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2023,6,2]],"date-time":"2023-06-02T00:00:00Z","timestamp":1685664000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100000001","name":"National Science Foundation","doi-asserted-by":"publisher","award":["2153790"],"award-info":[{"award-number":["2153790"]}],"id":[{"id":"10.13039\/100000001","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cytoskeleton"],"published-print":{"date-parts":[[2023,7]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Oocytes must be exceptionally large cells in order to support embryonic development. Throughout animal phylogeny, a specialized cell called a syncytium, wherein many nuclei share a continuous cytoplasm, achieves oogenesis. The syncytial nature of germline architecture is key to its function and depends on conserved components of the cortical cytoskeleton. Septins form non\u2010polar cytoskeletal polymers that associate with membranes. In the syncytial germline of the nematode\n                    <jats:italic>Caenorhabditis elegans<\/jats:italic>\n                    , septins are highly enriched on the cortex and generally required for fertility, but the role of septins in the germline is poorly understood. We report that the\n                    <jats:italic>C. elegans<\/jats:italic>\n                    septins, UNC\u201059 and UNC\u201061, are important for germline extension during development, the maintenance of its syncytial architecture, and production of oocytes. While much of our findings substantiate the idea that the two\n                    <jats:italic>C. elegans<\/jats:italic>\n                    septins act together, we also found evidence that they have distinct functions. Loss of UNC\u201061 perturbed germline extension during germline development, while the loss of UNC\u201059 function severely affected germline architecture in adult hermaphrodites. Consultation of clustering results from a large\u2010scale high\u2010throughput screen suggested that septins are involved in germ cell proliferation and\/or differentiation. In sum, our findings implicate a conserved cytoskeletal component in the complex architecture of a germline syncytium.\n                  <\/jats:p>","DOI":"10.1002\/cm.21763","type":"journal-article","created":{"date-parts":[[2023,6,2]],"date-time":"2023-06-02T10:38:30Z","timestamp":1685702310000},"page":"215-227","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["<i>Caenorhabditis elegans<\/i>\n                    septins contribute to the development and structure of the oogenic germline"],"prefix":"10.1002","volume":"80","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4342-7891","authenticated-orcid":false,"given":"Jenna A.","family":"Perry","sequence":"first","affiliation":[{"name":"Department of Biology University of North Carolina at Chapel Hill  Chapel Hill North Carolina USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-8729-0377","authenticated-orcid":false,"given":"Michael E.","family":"Werner","sequence":"additional","affiliation":[{"name":"Department of Biology University of North Carolina at Chapel Hill  Chapel Hill North Carolina USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-5946-8216","authenticated-orcid":false,"given":"Larry","family":"Rivenbark","sequence":"additional","affiliation":[{"name":"Department of Biology University of North Carolina at Chapel Hill  Chapel Hill North Carolina USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4671-2949","authenticated-orcid":false,"given":"Amy Shaub","family":"Maddox","sequence":"additional","affiliation":[{"name":"Department of Biology University of North Carolina at Chapel Hill  Chapel Hill North Carolina USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,6,2]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.201310117"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.199633"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-019-08344-5"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.E20-05-0304"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1093\/genetics\/77.1.71"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1314138111"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.201807211"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.17912\/micropub.biology.000200"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0912704107"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bpj.2022.12.026"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.e06-03-0170"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.120.10.2901"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbior.2019.100635"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.201405050"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.201006031"},{"key":"e_1_2_8_17_1","volume-title":"WormBook: The online review of C. elegans Biology [Internet]","author":"Fay D. S.","year":"2018"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0012-1606(03)00296-3"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2015.02.054"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.3390\/jdb8030014"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.E17-08-0502"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2011.03.037"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.E16-01-0063"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/0012-1606(76)90267-0"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0177(200005)218:1<2::AID-DVDY2>3.0.CO;2-W"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1534\/genetics.119.300238"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.emboj.7601775"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.01002"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1895\/wormbook.1.13.1"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/0012-1606(79)90035-6"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1002\/dvdy.22268"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.36919"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.01828"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.201706021"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcell.2021.793920"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.1006\/dbio.1998.9109"},{"key":"e_1_2_8_37_1","doi-asserted-by":"publisher","DOI":"10.1002\/cm.21569"},{"key":"e_1_2_8_38_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcell.2017.00003"},{"key":"e_1_2_8_39_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.113.21.3825"},{"key":"e_1_2_8_40_1","doi-asserted-by":"publisher","DOI":"10.15252\/embr.201541612"},{"key":"e_1_2_8_41_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4614-4015-4_1"},{"key":"e_1_2_8_42_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-018-07149-2"},{"key":"e_1_2_8_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2017.01.058"},{"key":"e_1_2_8_44_1","volume-title":"C. elegans II","author":"Riddle D. L.","year":"1997"},{"key":"e_1_2_8_45_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.2019"},{"key":"e_1_2_8_46_1","doi-asserted-by":"publisher","DOI":"10.1002\/wdev.87"},{"key":"e_1_2_8_47_1","doi-asserted-by":"publisher","DOI":"10.1534\/genetics.117.300082"},{"key":"e_1_2_8_48_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature06052"},{"key":"e_1_2_8_49_1","doi-asserted-by":"publisher","DOI":"10.15252\/embr.201541832"},{"key":"e_1_2_8_50_1","unstructured":"Smith L. I.(2018).Using quantitative fluorescent microscopy to characterize Centromeric epigenetic regulation in the developing embryo of Caenorhabditis elegans. Dissertation University of North Carolina at Chapel Hill.https:\/\/www.proquest.com\/pqdtlocal1006001\/docview\/2235934738\/610504A023AD4327PQ\/8?accountid=14244"},{"key":"e_1_2_8_51_1","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.E20-06-0398"},{"key":"e_1_2_8_52_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.004952"},{"key":"e_1_2_8_53_1","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.E20-09-0576"},{"key":"e_1_2_8_54_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10142-019-00690-3"}],"container-title":["Cytoskeleton"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cm.21763","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/cm.21763","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/am-pdf\/10.1002\/cm.21763","content-type":"application\/pdf","content-version":"am","intended-application":"syndication"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cm.21763","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,17]],"date-time":"2026-04-17T13:04:28Z","timestamp":1776431068000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/cm.21763"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,6,2]]},"references-count":53,"journal-issue":{"issue":"7-8","published-print":{"date-parts":[[2023,7]]}},"alternative-id":["10.1002\/cm.21763"],"URL":"https:\/\/doi.org\/10.1002\/cm.21763","archive":["Portico"],"relation":{},"ISSN":["1949-3584","1949-3592"],"issn-type":[{"value":"1949-3584","type":"print"},{"value":"1949-3592","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,6,2]]},"assertion":[{"value":"2022-12-01","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-05-04","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-06-02","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}