{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,12]],"date-time":"2026-02-12T07:52:22Z","timestamp":1770882742629,"version":"3.50.1"},"reference-count":53,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2023,12,23]],"date-time":"2023-12-23T00:00:00Z","timestamp":1703289600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000057","name":"National Institute of General Medical Sciences","doi-asserted-by":"publisher","award":["R01GM138652"],"award-info":[{"award-number":["R01GM138652"]}],"id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000062","name":"National Institute of Diabetes and Digestive and Kidney Diseases","doi-asserted-by":"publisher","award":["R01DK083345"],"award-info":[{"award-number":["R01DK083345"]}],"id":[{"id":"10.13039\/100000062","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cytoskeleton"],"published-print":{"date-parts":[[2024,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Metastasis of breast cancer cells to distant tissue sites is responsible for the majority of deaths associated with breast cancer. Previously we have examined the role of class I myosin motor protein, myosin 1e (myo1e), in cancer metastasis using the Mouse Mammary Tumor Virus\u2010Polyoma Middle T Antigen (MMTV\u2010PyMT) mouse model. Mice deficient in myo1e formed tumors with a more differentiated phenotype relative to the wild\u2010type mice and formed no detectable lung metastases. In the current study, we investigated how the absence of myo1e affects cell migration and invasion in vitro, using the highly invasive and migratory breast cancer cell line, 4T1. 4T1 cells deficient in myo1e exhibited an altered morphology and slower rates of migration in the wound\u2010healing and transwell migration assays compared to the WT 4T1 cells. While integrin trafficking and Golgi reorientation did not appear to be altered upon myo1e loss, we observed lower rates of focal adhesion disassembly in myo1e\u2010deficient cells, which could help explain the cell migration defect.<\/jats:p>","DOI":"10.1002\/cm.21819","type":"journal-article","created":{"date-parts":[[2023,12,23]],"date-time":"2023-12-23T11:53:48Z","timestamp":1703332428000},"page":"723-736","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Myosin 1e deficiency affects migration of <scp>4T1<\/scp> breast cancer cells"],"prefix":"10.1002","volume":"81","author":[{"given":"Michael E.","family":"Garone","sequence":"first","affiliation":[{"name":"Department of Cell and Developmental Biology State University of New York Upstate Medical University  Syracuse New York USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sharon E.","family":"Chase","sequence":"additional","affiliation":[{"name":"Department of Cell and Developmental Biology State University of New York Upstate Medical University  Syracuse New York USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chunling","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Neuroscience and Physiology State University of New York Upstate Medical University  Syracuse New York USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7008-9069","authenticated-orcid":false,"given":"Mira","family":"Krendel","sequence":"additional","affiliation":[{"name":"Department of Cell and Developmental Biology State University of New York Upstate Medical University  Syracuse New York USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2023,12,23]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1042\/EBC20190026"},{"key":"e_1_2_9_3_1","first-page":"1399","article-title":"Selective events in the metastatic process defined by analysis of the sequential dissemination of subpopulations of a mouse mammary tumor","volume":"52","author":"Aslakson C. J.","year":"1992","journal-title":"Cancer Research"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.12688\/f1000research.2-68.v1"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.00223.2013"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/cm.21162"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200805045"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btu170"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbior.2017.09.005"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.cellbio.20.012103.094243"},{"key":"e_1_2_9_11_1","doi-asserted-by":"crossref","DOI":"10.1242\/jcs.225029","article-title":"MYO1C stabilizes actin and facilitates the arrival of transport carriers at the Golgi complex","volume":"132","author":"Capmany A.","year":"2019","journal-title":"Journal of Cell Science"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.febslet.2009.03.037"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.e11-04-0383"},{"key":"e_1_2_9_14_1","first-page":"3174","article-title":"Heterogeneity of tumor cells from a single mouse mammary tumor","volume":"38","author":"Dexter D. L.","year":"1978","journal-title":"Cancer Research"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1080\/19336918.2021.2020705"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncb1262"},{"key":"e_1_2_9_17_1","unstructured":"Ferreira T. &Rasband W.(2012).ImageJ User Guide: IJ1.46r."},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.235275"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1242\/bio.20135827"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep00227"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2011.02.013"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1614894114"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2004.08.055"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcell.2021.640972"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0854.2012.01368.x"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.febslet.2007.01.021"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.4161\/sgtp.27958"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9440(10)63568-7"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1681\/ASN.2021111505"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1002\/dvdy.21658"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2015.11.012"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.198614"},{"key":"e_1_2_9_33_1","article-title":"Quantitative measurement of invadopodia\u2010mediated extracellular matrix proteolysis in single and multicellular contexts","author":"Martin K. H.","year":"2012","journal-title":"Journal of Visualized Experiments"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1007\/164_2016_29"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tcb.2010.04.004"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.119727"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41556-018-0223-z"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0901641106"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yexcr.2014.01.015"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.10139"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btp616"},{"key":"e_1_2_9_42_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200501162"},{"key":"e_1_2_9_43_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.202000740RR"},{"key":"e_1_2_9_44_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.2019"},{"key":"e_1_2_9_45_1","doi-asserted-by":"publisher","DOI":"10.3389\/fonc.2020.01195"},{"key":"e_1_2_9_46_1","first-page":"311","article-title":"Breast cancer metastasis","volume":"9","author":"Scully O. J.","year":"2012","journal-title":"Cancer Genomics & Proteomics"},{"key":"e_1_2_9_47_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.014977"},{"key":"e_1_2_9_48_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200502053"},{"key":"e_1_2_9_49_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2006.05.008"},{"key":"e_1_2_9_50_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.201503123"},{"key":"e_1_2_9_51_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncb1094"},{"key":"e_1_2_9_52_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tranon.2020.100899"},{"key":"e_1_2_9_53_1","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.E16-01-0005"},{"key":"e_1_2_9_54_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20100433"}],"container-title":["Cytoskeleton"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cm.21819","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,12,4]],"date-time":"2024-12-04T11:01:02Z","timestamp":1733310062000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/cm.21819"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,12,23]]},"references-count":53,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2024,12]]}},"alternative-id":["10.1002\/cm.21819"],"URL":"https:\/\/doi.org\/10.1002\/cm.21819","archive":["Portico"],"relation":{},"ISSN":["1949-3584","1949-3592"],"issn-type":[{"value":"1949-3584","type":"print"},{"value":"1949-3592","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,12,23]]},"assertion":[{"value":"2023-08-08","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-12-01","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-12-23","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}