{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,9]],"date-time":"2026-01-09T13:39:23Z","timestamp":1767965963016,"version":"3.49.0"},"reference-count":22,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2024,2,7]],"date-time":"2024-02-07T00:00:00Z","timestamp":1707264000000},"content-version":"am","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"},{"start":{"date-parts":[[2024,2,7]],"date-time":"2024-02-07T00:00:00Z","timestamp":1707264000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R01 EB030031"],"award-info":[{"award-number":["R01 EB030031"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["NIH NIBIB T32 EB005582"],"award-info":[{"award-number":["NIH NIBIB T32 EB005582"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000001","name":"National Science Foundation","doi-asserted-by":"publisher","award":["EF1935265"],"award-info":[{"award-number":["EF1935265"]}],"id":[{"id":"10.13039\/100000001","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000001","name":"National Science Foundation","doi-asserted-by":"publisher","award":["MCB\u20102201236"],"award-info":[{"award-number":["MCB\u20102201236"]}],"id":[{"id":"10.13039\/100000001","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cytoskeleton"],"published-print":{"date-parts":[[2024,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Although diverse actin network architectures found inside the cell have been individually reconstituted outside of the cell, how different types of actin architectures reorganize under applied forces is not entirely understood. Recently, bottom\u2010up reconstitution has enabled studies where dynamic and phenotypic characteristics of various actin networks can be recreated in an isolated cell\u2010like environment. Here, by creating a giant unilamellar vesicle (GUV)\u2010based cell model encapsulating actin networks, we investigate how actin networks rearrange in response to localized stresses applied by micropipette aspiration. We reconstitute actin bundles and branched bundles in GUVs separately and mechanically perturb them. Interestingly, we find that, when aspirated, protrusive actin bundles that are otherwise randomly oriented in the GUV lumen collapse and align along the axis of the micropipette. However, when branched bundles are aspirated, the network remains intact and outside of the pipette while the GUV membrane is aspirated into the micropipette. These results reveal distinct responses in the rearrangement of actin networks in a network architecture\u2010dependent manner when subjected to physical forces.<\/jats:p>","DOI":"10.1002\/cm.21836","type":"journal-article","created":{"date-parts":[[2024,2,8]],"date-time":"2024-02-08T05:19:22Z","timestamp":1707369562000},"page":"310-317","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Rearrangement of <scp>GUV<\/scp>\u2010confined actin networks in response to micropipette aspiration"],"prefix":"10.1002","volume":"81","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8735-8854","authenticated-orcid":false,"given":"Nadab H.","family":"Wubshet","sequence":"first","affiliation":[{"name":"Department of Mechanical Engineering University of Michigan  Ann Arbor Michigan United States"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cole J.","family":"Young","sequence":"additional","affiliation":[{"name":"Department of Mechanical Engineering University of Michigan  Ann Arbor Michigan United States"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0309-7018","authenticated-orcid":false,"given":"Allen P.","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Mechanical Engineering University of Michigan  Ann Arbor Michigan United States"},{"name":"Department of Biomedical Engineering University of Michigan  Ann Arbor Michigan United States"},{"name":"Cellular and Molecular Biology Program University of Michigan  Ann Arbor Michigan United States"},{"name":"Department of Biophysics University of Michigan  Ann Arbor Michigan United States"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,2,7]]},"reference":[{"issue":"177","key":"e_1_2_9_2_1","article-title":"Rapid encapsulation of reconstituted cytoskeleton inside giant unilamellar vesicles","author":"Bashirzadeh Y.","year":"2021","journal-title":"Journal of Visualized Experiments"},{"key":"e_1_2_9_3_1","first-page":"7","article-title":"Confinement geometry tunes fascin\u2010actin bundle structures and consequently the shape of a lipid bilayer vesicle","volume":"9","author":"Bashirzadeh Y.","year":"2020","journal-title":"Frontiers in Molecular 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