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The ciliary length is tightly regulated during ciliogenesis, but the impact of inflammation on ciliary length remains elusive. The current study investigates the outcome of inflammatory stimuli for the primary cilium length in retinal epithelium cells and mouse embryonic fibroblasts. Here, we report that exposure to the pro\u2010inflammatory cytokine TNF\u2010alpha elongates cilia in a mixed\u2010lineage kinase (MLK)\u2010dependent manner. Pro\u2010inflammatory stimuli such as bacterial LPS and interferon\u2010gamma have similar effects on ciliary length. In contrast, febrile condition\u2010mimicking heat stress dramatically reduced the number of ciliated cells regardless of TNF\u2010alpha exposure but did not shorten TNF\u2010induced elongation, suggesting distinct but rapid effects of inflammatory stresses on ciliogenesis.<\/jats:p>","DOI":"10.1002\/cm.21873","type":"journal-article","created":{"date-parts":[[2024,5,20]],"date-time":"2024-05-20T05:44:01Z","timestamp":1716183841000},"page":"639-647","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["<scp>TNF<\/scp>\n                    \u2010alpha promotes cilia elongation via mixed lineage kinases signaling in mouse fibroblasts and human\n                    <scp>RPE<\/scp>\n                    \u20101 cells"],"prefix":"10.1002","volume":"81","author":[{"given":"Amrita","family":"Kumari","sequence":"first","affiliation":[{"name":"Molecular, Cell and Cancer Biology Department University of Massachusetts Chan Medical School  Worcester Massachusetts USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amada D.","family":"Caliz","sequence":"additional","affiliation":[{"name":"Division of Cardiovascular Medicine, Department of Medicine Brigham and Women's Hospital, Harvard Medical School  Boston Massachusetts USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hyung\u2010Jin","family":"Yoo","sequence":"additional","affiliation":[{"name":"Division of Cardiovascular Medicine, Department of Medicine Brigham and Women's Hospital, Harvard Medical School  Boston Massachusetts USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shashi","family":"Kant","sequence":"additional","affiliation":[{"name":"Division of Cardiovascular Medicine, Department of Medicine Brigham and Women's Hospital, Harvard Medical School  Boston Massachusetts USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0939-0389","authenticated-orcid":false,"given":"Anastassiia","family":"Vertii","sequence":"additional","affiliation":[{"name":"Molecular, Cell and Cancer Biology Department University of Massachusetts Chan Medical School  Worcester Massachusetts USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,5,20]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ceb.2012.08.013"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12974-017-0958-7"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/humu.22618"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.4161\/cc.7.7.5610"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.98.3.847"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.271.2.833"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.202303052"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbadis.2016.05.022"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1177\/2045894018764629"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.239871"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrm906"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12929-019-0580-3"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1681\/ASN.2011080829"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2013.07.019"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1101\/gad.17224711"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41420-022-01272-2"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4827(91)90365-2"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tcb.2023.05.005"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1093\/jnci\/59.4.1263"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2017.12.038"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.ade9459"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncb1529"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1002\/pul2.12167"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.100545"},{"key":"e_1_2_8_26_1","first-page":"62","article-title":"c\u2010JUN n\u2010terminal kinase (JNK) signaling in autosomal dominant polycystic kidney disease","volume":"3","author":"Smith A. 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