{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,14]],"date-time":"2025-11-14T07:40:29Z","timestamp":1763106029276,"version":"3.33.0"},"reference-count":33,"publisher":"Wiley","issue":"1-2","license":[{"start":{"date-parts":[[2024,7,12]],"date-time":"2024-07-12T00:00:00Z","timestamp":1720742400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cytoskeleton"],"published-print":{"date-parts":[[2025,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The serine\/threonine kinase polo\u2010like kinase 1 (PLK1) is a master regulator of cell proliferation and contraction, but its physiological role in the lower urinary tract is unknown. We utilized transcriptomic programs of human bladder smooth muscle cells (hBSMCs), 3D bladder spheroid viability assays, and human ureterovesical junction contractility measurements to elucidate the impacts of PLK1 inhibition. This work reveals PLK1 reduction with the selective inhibitor TAK\u2010960 (500\u2009nM) suppresses high K+\u2010evoked contractions of human urinary smooth muscle ex vivo while decreasing urothelial cell viability. Transcriptomic analysis of hBSMCs treated with TAK\u2010960 shows modulation of cell cycle and contraction pathways, specifically through altered expression of Cys2\/His2\u2010type zinc finger transcription factors. In bladder spheroids, PLK1 inhibition also suppresses smooth muscle contraction protein filamin. Taken together, these findings establish PLK1 is a critical governor of urinary smooth muscle contraction and urothelial proliferation with implications for lower urinary tract disorders. Targeting PLK1 pharmacologically may therefore offer therapeutic potential to ameliorate hypercontractility and aberrant growth. Further elucidation of PLK1 signaling networks promises new insights into pathogenesis and much needed treatment advances for debilitating urinary symptoms.<\/jats:p>","DOI":"10.1002\/cm.21888","type":"journal-article","created":{"date-parts":[[2024,7,12]],"date-time":"2024-07-12T11:45:18Z","timestamp":1720784718000},"page":"58-70","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Polo\u2010like kinase 1 inhibition modulates urinary tract smooth muscle contraction and bladder cell transcriptional programs"],"prefix":"10.1002","volume":"82","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6142-9722","authenticated-orcid":false,"given":"Xiaolong","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Urology Zhongnan Hospital of Wuhan University  Wuhan 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Wuhan China"},{"name":"Institute of Urology Wuhan University  Wuhan China"},{"name":"Hubei Medical Quality Control Center for Laparoscopic\/Endoscopic Urologic Surgery  Wuhan 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