{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,6,10]],"date-time":"2025-06-10T04:01:16Z","timestamp":1749528076410,"version":"3.41.0"},"reference-count":22,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2024,10,10]],"date-time":"2024-10-10T00:00:00Z","timestamp":1728518400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000038","name":"Natural Sciences and Engineering Research Council of Canada","doi-asserted-by":"publisher","award":["RGPIN\u20102018\u201003727"],"award-info":[{"award-number":["RGPIN\u20102018\u201003727"]}],"id":[{"id":"10.13039\/501100000038","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cytoskeleton"],"published-print":{"date-parts":[[2025,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Myosin VI has been reported by others to localize in association with various regions of apical tubulobulbar complexes (TBCs) at sites of attachment between Sertoli cells and late spermatids in the mouse. Tubulobulbar complexes internalize \u201cintact\u201d intercellular junctions during sperm release and during spermatocyte translocation through the blood\u2010testis barrier. Here, we use super\u2010resolution (STED\u2014stimulated emission depletion) and electron microscopy of immunolabeled sections of rat testis to clearly define the localization of anti\u2010myosin VI reactivity both at apical and basal sites in the epithelium. In data stacks collected by STED imaging, staining at TBCs was predominantly associated with bulb regions of the complexes. At apical sites, when data stacks were analyzed with an Imaris software, staining appeared around and extended between adjacent bulbs. At basal sites, in addition to labeling at TBC bulbs, reactive sites appeared concentrated in regions close to but not directly associated with intercellular junctions. At the ultrastructural level, labeling was predominantly associated with cisternae of the endoplasmic reticulum associated with the bulbs of TBCs and near to basal junction complexes. We conclude that myosin VI may be associated with specific subdomains of the endoplasmic reticulum related to TBC bulbs and associated basal junction complexes between Sertoli cells.<\/jats:p>","DOI":"10.1002\/cm.21949","type":"journal-article","created":{"date-parts":[[2024,10,11]],"date-time":"2024-10-11T04:29:03Z","timestamp":1728620943000},"page":"333-343","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Myosin <scp>VI<\/scp> Is Associated With the Endoplasmic Reticulum in Regions of Sertoli Cells Containing Tubulobulbar Complexes"],"prefix":"10.1002","volume":"82","author":[{"given":"Samuel","family":"Tretjakov","sequence":"first","affiliation":[{"name":"Life Sciences Centre and the Department of Cellular and Physiological Sciences The University of British Columbia  Vancouver British Columbia Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Prunveer","family":"Palia","sequence":"additional","affiliation":[{"name":"Life Sciences Centre and the Department of Cellular and Physiological Sciences The University of British Columbia  Vancouver British Columbia Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7694-7602","authenticated-orcid":false,"given":"A. Wayne","family":"Vogl","sequence":"additional","affiliation":[{"name":"Life Sciences Centre and the Department of Cellular and Physiological Sciences The University of British Columbia  Vancouver British Columbia Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,10,10]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1093\/biolre\/ioz120"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/ar.1091960109"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/1873\u20103468.13486"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.258785"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.10.12.4341"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.3000211"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/227680a0"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1093\/biolre\/iox042"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod.115.128942"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.sbi.2020.09.005"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2014.163"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1093\/biolre\/ioab161"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.201401126"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/ar.1091850302"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/ar.1091940204"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/0040-8166(80)90005-1"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00018\u2010015\u20102052\u20106"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1530\/REP\u201017\u20100281"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1038\/46835"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00418\u2010020\u201001954\u2010x"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00418\u2010017\u20101579\u2010z"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1093\/biolre\/ioz232"}],"container-title":["Cytoskeleton"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cm.21949","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,6,9]],"date-time":"2025-06-09T08:02:00Z","timestamp":1749456120000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/cm.21949"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,10,10]]},"references-count":22,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2025,6]]}},"alternative-id":["10.1002\/cm.21949"],"URL":"https:\/\/doi.org\/10.1002\/cm.21949","archive":["Portico"],"relation":{},"ISSN":["1949-3584","1949-3592"],"issn-type":[{"type":"print","value":"1949-3584"},{"type":"electronic","value":"1949-3592"}],"subject":[],"published":{"date-parts":[[2024,10,10]]},"assertion":[{"value":"2024-07-03","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-10-02","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-10-10","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}