{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T17:31:48Z","timestamp":1787160708369,"version":"build-2736575974"},"reference-count":34,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2025,3,12]],"date-time":"2025-03-12T00:00:00Z","timestamp":1741737600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100011796","name":"Mount Saint Vincent University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100011796","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000038","name":"Natural Sciences and Engineering Research Council of Canada","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000038","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000288","name":"Royal Society","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000288","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100020170","name":"Research Nova Scotia","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100020170","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000196","name":"Canada Foundation for Innovation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000196","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cytoskeleton"],"published-print":{"date-parts":[[2025,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    The organization of microtubules into a mitotic spindle is critical for animal cell proliferation and involves the cooperation of hundreds of proteins whose molecular roles and regulation are not fully understood. The protein product of the\n                    <jats:italic>Drosophila<\/jats:italic>\n                    gene\n                    <jats:italic>abnormal spindle<\/jats:italic>\n                    , Asp, is a microtubule\u2010associated protein required for correct mitotic spindle formation. To better understand the contribution of Asp to microtubule organization during spindle formation, we reverse\u2010engineered flies to express a version of Asp (Asp\n                    <jats:sup>LIE<\/jats:sup>\n                    ), predicted to have lost its ability to bind the phosphatase trimer PP2A\u2010B56. We demonstrated that the Asp\n                    <jats:sup>LIE<\/jats:sup>\n                    mutation reduced an interaction with the\n                    <jats:italic>Drosophila<\/jats:italic>\n                    PP2A\u2010B56 regulatory subunit Widerborst (Wdb), as well as other proteins with known roles in spindle formation. Asp\n                    <jats:sup>LIE<\/jats:sup>\n                    flies exhibited less robust microtubule minus\u2010end cohesion at neural stem cell spindle poles, which was accompanied by a substantial developmental delay but no microcephaly. Predictive structural modeling suggests that the presence of Wdb alters the conformation of an Asp interaction with a tubulin dimer in a manner similar to that of the Asp\n                    <jats:sup>LIE<\/jats:sup>\n                    mutation. Protein localization in the\n                    <jats:italic>Drosophila<\/jats:italic>\n                    embryo, in addition to in\u00a0vitro microtubule organization experiments, suggests that a role of PP2A may be to prevent Asp from contributing to microtubule cross\u2010linking at spindle microtubule plus ends. Together, these findings add new insights to mechanisms underlying microtubule organization within the mitotic spindle.\n                  <\/jats:p>","DOI":"10.1002\/cm.22013","type":"journal-article","created":{"date-parts":[[2025,3,12]],"date-time":"2025-03-12T08:40:36Z","timestamp":1741768836000},"page":"804-814","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["The\n                    <scp>PP2A<\/scp>\n                    \u2010\n                    <scp>B56<\/scp>\n                    Binding Site\n                    <scp>LxxIxE<\/scp>\n                    Contributes to Asp\u2010Mediated Spindle Pole Stability"],"prefix":"10.1002","volume":"82","author":[{"given":"Margaux","family":"Quiniou","sequence":"first","affiliation":[{"name":"Department of Biosciences University of Exeter, Living Systems Institute  Exeter UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria C.","family":"Burns","sequence":"additional","affiliation":[{"name":"Biology Department Mount Saint Vincent University  Halifax Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aynsley","family":"McDermott","sequence":"additional","affiliation":[{"name":"Biology Department Mount Saint Vincent University  Halifax Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karolina","family":"Jaworek","sequence":"additional","affiliation":[{"name":"Department of Biosciences University of Exeter, Living Systems Institute  Exeter UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stacey J.","family":"Scott","sequence":"additional","affiliation":[{"name":"Department of Biosciences University of Exeter, Living Systems Institute  Exeter UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James G.","family":"Wakefield","sequence":"additional","affiliation":[{"name":"Department of Biosciences University of Exeter, Living Systems Institute  Exeter UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lori","family":"Borgal","sequence":"additional","affiliation":[{"name":"Biology Department Mount Saint Vincent University  Halifax Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,3,12]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586\u2010024\u201007487\u2010w"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2007.01.068"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1242\/bio.022905"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkw1244"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncb2327"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1141314"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2013.12.001"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2016.06.024"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.201507001"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.254037"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.122481"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/humu.23381"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1008815"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/pro.4792"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.027599"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41592\u2010022\u201001488\u20101"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.3390\/biom9020055"},{"key":"e_1_2_10_19_1","doi-asserted-by":"crossref","unstructured":"Naumov V. I.Balashov V.Lagutin P.Borovikov andA.Alexeev.2017.\u201cVolcanoR \u2010 Web Service to Produce Volcano Plots and Do Basic Enrichment Analysis.\u201d165100. bioRxiv.https:\/\/doi.org\/10.1101\/165100.","DOI":"10.1101\/165100"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2015.05.033"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.236786"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.40325"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btl022"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.3390\/cells12060922"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncb2858"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.137.4.881"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.2019"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.201509054"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1186\/2046\u20102530\u20103\u20106"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2012.09.005"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.49769"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.153.4.637"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13238\u2010016\u20100283\u20104"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.1038\/celldisc.2017.27"},{"key":"e_1_2_10_35_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcell.2020.00342"}],"container-title":["Cytoskeleton"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cm.22013","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,12,13]],"date-time":"2025-12-13T09:06:02Z","timestamp":1765616762000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/cm.22013"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,3,12]]},"references-count":34,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2025,12]]}},"alternative-id":["10.1002\/cm.22013"],"URL":"https:\/\/doi.org\/10.1002\/cm.22013","archive":["Portico"],"relation":{},"ISSN":["1949-3584","1949-3592"],"issn-type":[{"value":"1949-3584","type":"print"},{"value":"1949-3592","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,3,12]]},"assertion":[{"value":"2024-11-01","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-03-03","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-03-12","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}