{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T06:59:17Z","timestamp":1789714757034,"version":"4.0.1"},"reference-count":27,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T00:00:00Z","timestamp":1753833600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T00:00:00Z","timestamp":1753833600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100018693","name":"HORIZON EUROPE Framework Programme","doi-asserted-by":"publisher","award":["772853"],"award-info":[{"award-number":["772853"]}],"id":[{"id":"10.13039\/100018693","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cytoskeleton"],"published-print":{"date-parts":[[2026,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Septins are conserved GTP\u2010binding proteins that play key roles in cell division, mitochondrial dynamics and immune responses. Despite their importance to human health, pharmacological compounds to modify septins remain limited. Forchlorfenuron (FCF) was the first small molecule identified to modify septins, disrupting their organisation and promoting mitochondrial fragmentation. A more potent FCF analog (UR214\u20109) has recently been developed, but its effects on mitochondria were unknown. Here, we compare FCF and UR214\u20109 in\u00a0vitro using macrophages and in\u00a0vivo using zebrafish larvae. We demonstrate that both modifiers induce mitochondrial fragmentation in macrophages without altering mitochondrial mass or SEPT7 expression. Consistent with mitochondrial fragmentation, both modifiers trigger lytic cell death in a dose\u2010dependent manner following lipopolysaccharide (LPS) priming. In\u00a0vivo, both modifiers exhibit dose\u2010dependent effects on the survival of zebrafish larvae, although UR214\u20109 was significantly more toxic. In agreement with in\u00a0vitro results, we observed that FCF induces macrophage cell death and caspase\u20101 activity in zebrafish larvae. Together, our findings show that both septin modifiers impact mitochondrial integrity and macrophage survival. Understanding how septin modifiers regulate immune responses may have important implications for inflammatory disease research and could lead to the development of septin\u2010based medicines for conditions characterised by dysregulated inflammation.<\/jats:p>","DOI":"10.1002\/cm.70013","type":"journal-article","created":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T09:36:17Z","timestamp":1753868177000},"page":"179-186","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Comparative Analysis of Septin Modifiers, Forchlorfenuron and\n                    <scp>UR214<\/scp>\n                    \u20109, on Mitochondrial Fragmentation and Lytic Cell Death"],"prefix":"10.1002","volume":"83","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6304-3292","authenticated-orcid":false,"given":"Dominik","family":"Brokatzky","sequence":"first","affiliation":[{"name":"Department of Infection Biology London School of Hygiene &amp; Tropical Medicine  London UK"},{"name":"Institut Pasteur, Universit\u00e9 Paris Cit\u00e9, CNRS UMR3691 Dynamics of Host\u2010Pathogen Interactions Unit  Paris France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rajdeep","family":"Das","sequence":"additional","affiliation":[{"name":"Department of Infection Biology London School of Hygiene &amp; Tropical Medicine  London UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hannah","family":"Painter","sequence":"additional","affiliation":[{"name":"Department of Infection Biology London School of Hygiene &amp; Tropical Medicine  London UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rakesh K.","family":"Singh","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology University of Rochester Medical Center  Rochester New York USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7286-6503","authenticated-orcid":false,"given":"Serge","family":"Mostowy","sequence":"additional","affiliation":[{"name":"Department of Infection Biology London School of Hygiene &amp; Tropical Medicine  London UK"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,7,30]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chembiol.2024.07.003"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1242\/dmm.049414"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.chemrestox.9b00335"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0966-842X(00)01936-3"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/cm.20452"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41418-017-0012-4"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.adf9706"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tim.2019.08.005"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1128\/EC.00191-14"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/bs.mcb.2016.03.013"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chembiol.2024.04.012"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M804962200"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1266\/ggs.79.199"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-020-59824-4"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biochi.2010.05.006"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bpj.2023.07.030"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1006467"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrm3284"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.15252\/embr.201541612"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20181892"},{"key":"e_1_2_10_22_1","first-page":"384","article-title":"Pharmacological Modulation of Septins Restores Calcium Homeostasis and Is Neuroprotective in Models of Alzheimer's Disease","volume":"1979","author":"Princen K.","year":"2024","journal-title":"Science"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.21273\/JASHS.117.1.85"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.15252\/embr.201541832"},{"key":"e_1_2_10_25_1","article-title":"Shigella Serotypes Associated With Carriage in Humans Establish Persistent Infection in Zebrafish","volume":"228","author":"Torraca V.","year":"2023","journal-title":"Journal of Infectious Diseases"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1002\/cm.21696"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-023-05758-6"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.adi1788"}],"container-title":["Cytoskeleton"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cm.70013","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/cm.70013","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cm.70013","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,15]],"date-time":"2026-04-15T04:31:42Z","timestamp":1776227502000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/cm.70013"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,7,30]]},"references-count":27,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2026,4]]}},"alternative-id":["10.1002\/cm.70013"],"URL":"https:\/\/doi.org\/10.1002\/cm.70013","archive":["Portico"],"relation":{},"ISSN":["1949-3584","1949-3592"],"issn-type":[{"value":"1949-3584","type":"print"},{"value":"1949-3592","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,7,30]]},"assertion":[{"value":"2025-04-07","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-07-18","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-07-30","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}