{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T04:20:17Z","timestamp":1790742017727,"version":"4.1.0"},"reference-count":32,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2025,8,2]],"date-time":"2025-08-02T00:00:00Z","timestamp":1754092800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,8,2]],"date-time":"2025-08-02T00:00:00Z","timestamp":1754092800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100007129","name":"Natural Science Foundation of Shandong Province","doi-asserted-by":"publisher","award":["ZR2024MC136"],"award-info":[{"award-number":["ZR2024MC136"]}],"id":[{"id":"10.13039\/501100007129","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cytoskeleton"],"published-print":{"date-parts":[[2026,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    In animal cells, centrosomes function as the microtubule\u2010organizing centers; their presence is essential for mitosis and for assembling various cilia\u2014both primary and motile. Here, we identified coiled\u2010coil domain containing 81 (CCDC81) located at the centrosome through its 301\u2013505\u2009aa. Using bioinformatics approaches, we constructed a Neighbor\u2010Joining phylogenetic tree. We also analyzed the conservation of the CCDC81 protein sequence. The results reveal a high degree of conservation in mammals, implying a potentially vital biological role for CCDC81. Silencing of CCDC81 resulted in a decrease in both the frequency and length of primary cilia, yet it exerted no significant impact on centriole number. Examining CCDC81 tissue distribution in mice revealed markedly elevated\n                    <jats:italic>Ccdc81<\/jats:italic>\n                    mRNA levels in testis, lung, trachea, and fallopian tubes\u2014tissues characterized by abundant motile cilia.\n                    <jats:italic>Ccdc81<\/jats:italic>\n                    knockout mice were generated using CRISPR\/Cas9 technology. Over a six\u2010week period, body weight measurements of knockout mice showed no significant abnormalities. Our research results suggest that CCDC81 is indispensable for the formation of primary cilia and plays a role in the function of motile cilia.\n                  <\/jats:p>","DOI":"10.1002\/cm.70021","type":"journal-article","created":{"date-parts":[[2025,8,2]],"date-time":"2025-08-02T10:04:06Z","timestamp":1754129046000},"page":"570-577","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Centrosome Protein\n                    <scp>CCDC81<\/scp>\n                    Promotes Ciliogenesis"],"prefix":"10.1002","volume":"83","author":[{"given":"Xunshuo","family":"Liu","sequence":"first","affiliation":[{"name":"Center for Cell Structure and Function, Shandong Provincial Key Laboratory of Animal Resistance Biology College of Life Sciences, Shandong Normal University  Jinan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fangyuan","family":"Liu","sequence":"additional","affiliation":[{"name":"Center for Cell Structure and Function, Shandong Provincial Key Laboratory of Animal Resistance Biology College of Life Sciences, Shandong Normal University  Jinan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-9959-5300","authenticated-orcid":false,"given":"Shanshan","family":"Nai","sequence":"additional","affiliation":[{"name":"Center for Cell Structure and Function, Shandong Provincial Key Laboratory of Animal Resistance Biology College of Life Sciences, Shandong Normal University  Jinan China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,8,2]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.154500"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1093\/molehr\/gaab009"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.E18\u201002\u20100115"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.3390\/jcm8050619"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrm2180"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1209037"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2023.06.013"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.nbd.2022.105913"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00004.2021"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/ddt445"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/cddis.2013.108"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.201101005"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.semcdb.2020.11.007"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41419\u2010024\u201006428\u20109"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41392\u2010023\u201001722\u2010y"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2023.06.079"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.202307150"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2009.10.036"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCGEN.119.002686"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1183\/16000617.0106\u20102023"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1007\/s43032\u2010021\u201000595\u20102"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrm.2017.60"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1186\/1747\u20101028\u20105\u201025"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.semcdb.2021.03.020"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.203505"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.15252\/embr.201846735"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467\u2010021\u201021506\u20108"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.15252\/embr.202152775"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncb2880"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1093\/jmcb\/mjy014"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.E15\u201009\u20100655"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jgg.2024.07.010"}],"container-title":["Cytoskeleton"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cm.70021","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/cm.70021","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cm.70021","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T19:11:41Z","timestamp":1787080301000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/cm.70021"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,8,2]]},"references-count":32,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2026,8]]}},"alternative-id":["10.1002\/cm.70021"],"URL":"https:\/\/doi.org\/10.1002\/cm.70021","archive":["Portico"],"relation":{},"ISSN":["1949-3584","1949-3592"],"issn-type":[{"value":"1949-3584","type":"print"},{"value":"1949-3592","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,8,2]]},"assertion":[{"value":"2025-05-31","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication 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