{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,17]],"date-time":"2026-02-17T12:22:35Z","timestamp":1771330955555,"version":"3.50.1"},"reference-count":39,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,2,17]],"date-time":"2026-02-17T00:00:00Z","timestamp":1771286400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,2,17]],"date-time":"2026-02-17T00:00:00Z","timestamp":1771286400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82101464"],"award-info":[{"award-number":["82101464"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32471038"],"award-info":[{"award-number":["32471038"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32070725"],"award-info":[{"award-number":["32070725"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cytoskeleton"],"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Our understanding of the intrinsic mechanisms that drive the regeneration of damaged axons after a spinal cord injury is still limited. Microtubules are core components of the eukaryotic cytoskeleton and are essential for axonal growth, in part because their stability is governed by post\u2010translational modifications in mature neurons. Glucose\u2010dependent insulinotropic polypeptide (GIP) and its receptor (GIPR) are expressed in multiple extra\u2010pancreatic tissues, suggesting biological functions beyond classical endocrine signaling; however, their roles in neuronal cytoskeletal regulation are not well defined. Here, we investigated the effects of GIP in cultured cortical neurons. GIP enhanced microtubule stability and increased the number of axons crossing an inhibitory chondroitin sulfate proteoglycan (CSPG) border. Mechanistically, GIP promoted microtubule acetylation via \u03b1\u2010tubulin N\u2010acetyltransferase 1 (\u03b1TAT1), the major acetyltransferase for \u03b1\u2010tubulin, by suppressing \u03b1TAT1 ubiquitination and thereby reducing its proteasomal degradation in inhibitory environments. Although the upstream mechanism remains to be determined, this study provides the first evidence that GIP\/GIPR signaling modulates microtubule dynamics, highlighting a potential strategy to re\u2010activate neuronal growth machinery after injury.<\/jats:p>","DOI":"10.1002\/cm.70107","type":"journal-article","created":{"date-parts":[[2026,2,17]],"date-time":"2026-02-17T11:33:37Z","timestamp":1771328017000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["<scp>GIP<\/scp>\n                    Affects Neuronal Microtubule Acetylation by Regulating\n                    <scp>\u03b1TAT1<\/scp>\n                    Degradation"],"prefix":"10.1002","author":[{"given":"Beibei","family":"Guo","sequence":"first","affiliation":[{"name":"Jiangsu Key Laboratory of Tissue Engineering and Neuroregeneration, Key Laboratory of Neuroregeneration of Ministry of Education, co\u2010Innovation Center of Neuroregeneration Nantong University  Nantong Jiangsu China"},{"name":"Medical School of Nantong University  Nantong Jiangsu China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yan","family":"Yue","sequence":"additional","affiliation":[{"name":"Jiangsu Key Laboratory of Tissue Engineering and Neuroregeneration, Key Laboratory of Neuroregeneration of Ministry of Education, co\u2010Innovation Center of Neuroregeneration Nantong University  Nantong Jiangsu China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaoqian","family":"Luo","sequence":"additional","affiliation":[{"name":"Jiangsu Key Laboratory of Tissue Engineering and Neuroregeneration, Key Laboratory of Neuroregeneration of Ministry of Education, co\u2010Innovation Center of Neuroregeneration Nantong University  Nantong Jiangsu China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yao","family":"Zhou","sequence":"additional","affiliation":[{"name":"Jiangsu Key Laboratory of Tissue Engineering and Neuroregeneration, Key Laboratory of Neuroregeneration of Ministry of Education, co\u2010Innovation Center of Neuroregeneration Nantong University  Nantong Jiangsu China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jing","family":"Yu","sequence":"additional","affiliation":[{"name":"Jiangsu Key Laboratory of Tissue Engineering and Neuroregeneration, Key Laboratory of Neuroregeneration of Ministry of Education, co\u2010Innovation Center of Neuroregeneration Nantong University  Nantong Jiangsu China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ximan","family":"Yang","sequence":"additional","affiliation":[{"name":"Jiangsu Key Laboratory of Tissue Engineering and Neuroregeneration, Key Laboratory of Neuroregeneration of Ministry of Education, co\u2010Innovation Center of Neuroregeneration Nantong University  Nantong Jiangsu China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yufang","family":"Zhang","sequence":"additional","affiliation":[{"name":"Jiangsu Key Laboratory of Tissue Engineering and Neuroregeneration, Key Laboratory of Neuroregeneration of Ministry of Education, co\u2010Innovation Center of Neuroregeneration Nantong University  Nantong Jiangsu China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Man","family":"Xu","sequence":"additional","affiliation":[{"name":"Jiangsu Key Laboratory of Tissue Engineering and Neuroregeneration, Key Laboratory of Neuroregeneration of Ministry of Education, co\u2010Innovation Center of Neuroregeneration Nantong University  Nantong Jiangsu China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1168-6119","authenticated-orcid":false,"given":"Mei","family":"Liu","sequence":"additional","affiliation":[{"name":"Jiangsu Key Laboratory of Tissue Engineering and Neuroregeneration, Key Laboratory of Neuroregeneration of Ministry of Education, co\u2010Innovation Center of Neuroregeneration Nantong University  Nantong Jiangsu China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1378-1825","authenticated-orcid":false,"given":"Yan","family":"Liu","sequence":"additional","affiliation":[{"name":"Jiangsu Key Laboratory of Tissue Engineering and Neuroregeneration, Key Laboratory of Neuroregeneration of Ministry of Education, co\u2010Innovation Center of Neuroregeneration Nantong University  Nantong Jiangsu China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tuchen","family":"Guan","sequence":"additional","affiliation":[{"name":"Jiangsu Key Laboratory of Tissue Engineering and Neuroregeneration, Key Laboratory of Neuroregeneration of Ministry of Education, co\u2010Innovation Center of Neuroregeneration Nantong University  Nantong Jiangsu China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2026,2,17]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.225805"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.2254\u201004.2004"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471\u20102377\u20109\u201032"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmet.2013.04.008"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41583\u2010019\u20100196\u20103"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/jnc.15816"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1093\/ejendo\/lvac004"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.2337\/diabetes.51.3.652"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/417455a"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.3389\/fncel.2020.00208"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrm3227"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2017.10.044"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/jnr.23573"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13311\u2010013\u20100203\u20108"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.4103\/1673\u20105374.373716"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.201612104"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.21769\/BioProtoc.4690"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms14787"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.4920\u201004.2005"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep37152"},{"key":"e_1_2_11_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.expneurol.2021.113794"},{"key":"e_1_2_11_23_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.4885\u201010.2011"},{"key":"e_1_2_11_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.expneurol.2012.07.009"},{"key":"e_1_2_11_25_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1013728107"},{"key":"e_1_2_11_26_1","doi-asserted-by":"publisher","DOI":"10.1002\/dneu.22174"},{"key":"e_1_2_11_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tcb.2014.10.004"},{"key":"e_1_2_11_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2020.11.120"},{"key":"e_1_2_11_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2012.03.066"},{"key":"e_1_2_11_30_1","doi-asserted-by":"publisher","DOI":"10.3389\/fnmol.2012.00024"},{"key":"e_1_2_11_31_1","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00017.2017"},{"key":"e_1_2_11_32_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00441\u2010021\u201003477\u2010w"},{"key":"e_1_2_11_33_1","doi-asserted-by":"publisher","DOI":"10.1210\/endo.133.6.8243312"},{"key":"e_1_2_11_34_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00018\u2010016\u20102168\u20103"},{"key":"e_1_2_11_35_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.aau1504"},{"key":"e_1_2_11_36_1","doi-asserted-by":"publisher","DOI":"10.4161\/cc.6.17.4633"},{"key":"e_1_2_11_37_1","doi-asserted-by":"publisher","DOI":"10.1523\/ENEURO.0240\u201017.2018"},{"key":"e_1_2_11_38_1","doi-asserted-by":"publisher","DOI":"10.3389\/fncel.2018.00124"},{"key":"e_1_2_11_39_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.aai8764"},{"key":"e_1_2_11_40_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.01154\u201006"}],"container-title":["Cytoskeleton"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cm.70107","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/cm.70107","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cm.70107","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,2,17]],"date-time":"2026-02-17T11:33:37Z","timestamp":1771328017000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/cm.70107"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,2,17]]},"references-count":39,"alternative-id":["10.1002\/cm.70107"],"URL":"https:\/\/doi.org\/10.1002\/cm.70107","archive":["Portico"],"relation":{},"ISSN":["1949-3584","1949-3592"],"issn-type":[{"value":"1949-3584","type":"print"},{"value":"1949-3592","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,2,17]]},"assertion":[{"value":"2025-10-19","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-01-28","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-02-17","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"cm.70107"}}