{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,4]],"date-time":"2026-08-04T08:46:51Z","timestamp":1785833211066,"version":"3.56.0"},"reference-count":39,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,8,4]],"date-time":"2026-08-04T00:00:00Z","timestamp":1785801600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,8,4]],"date-time":"2026-08-04T00:00:00Z","timestamp":1785801600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100000069","name":"National Institute of Arthritis and Musculoskeletal and Skin Diseases","doi-asserted-by":"publisher","award":["R01\u2010AR\u201057063"],"award-info":[{"award-number":["R01\u2010AR\u201057063"]}],"id":[{"id":"10.13039\/100000069","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cytoskeleton"],"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>The technique of fluorescence resonance energy transfer (FRET) has been used in this study to determine the proximities (efficiencies, i.e., E%) of interacting proteins in sarcomeres in living cultured embryonic chicken skeletal muscle cells to draw up a FRETsome of a sarcomere in mature myofibrils. During this study, two known Z\u2010band proteins, FATZ (also called myozenin), and telethonin, were found to be actin binding proteins. The Efficiency of the F\u2010actin and telethonin pair was measured to be 15%, while the F\u2010actin and FATZ pair was 18%. The known F\u2010actin and alpha\u2013actinin interactions resulted in an efficiency of 13%. The new actin binding properties of FATZ and telethonin were confirmed by F\u2010actin pull down experiments. By placing a donor probe (CeFP) on one end of the FATZ molecule and the acceptor probe (YFP) on the other end of FATZ, we were able to determine that the two termini moved apart from each other as the dense bodies of the nascent myofibrils fused with one another to form the Z\u2010bands of mature myofibrils. Three other proteins of the dense bodies, i.e., myotilin, alpha\u2010actinin and ArgBP2 also underwent similar termini separation changes as the dense bodies transformed into Z\u2010bands of mature myofibrils. The highest efficiencies were measured for MyBP\u2010C interactions with myosin heavy chains (35%), myosin light chains (35%), and F\u2010actin (31%). The establishment of a sarcomere FRETsome of these interactions in control sarcomeres in mature myofibrils in living transfected skeletal muscles establishes a foundation on which the introduction of individual mutated sarcomeric proteins involved in different muscle diseases will have on their interactions with control partner sarcomeric proteins in mature myofibrils.<\/jats:p>","DOI":"10.1002\/cm.70169","type":"journal-article","created":{"date-parts":[[2026,8,4]],"date-time":"2026-08-04T08:21:10Z","timestamp":1785831670000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Determining Proximities of Sarcomeric Proteins in Living Skeletal Muscle Cells Using\n                    <scp>FRET<\/scp>\n                    Imaging"],"prefix":"10.1002","author":[{"ORCID":"https:\/\/orcid.org\/0009-0005-9800-074X","authenticated-orcid":false,"given":"Jushuo","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Cell and Developmental Biology SUNY Upstate Medical University  Syracuse New York USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-6216-9475","authenticated-orcid":false,"given":"Yingli","family":"Fan","sequence":"additional","affiliation":[{"name":"Department of Cell and Developmental Biology SUNY Upstate Medical University  Syracuse New York USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-5700-7373","authenticated-orcid":false,"given":"Vasundhara","family":"Muthu","sequence":"additional","affiliation":[{"name":"Department of Medicine SUNY Upstate Medical University  Syracuse New York USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1179-7073","authenticated-orcid":false,"given":"Symalima","family":"Dube","sequence":"additional","affiliation":[{"name":"Department of Medicine SUNY Upstate Medical University  Syracuse New York USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-0964-6905","authenticated-orcid":false,"given":"Naruki","family":"Sato","sequence":"additional","affiliation":[{"name":"Department of Biology, Graduate School of Science Chiba University  Chiba Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0205-4761","authenticated-orcid":false,"given":"Dipak K.","family":"Dube","sequence":"additional","affiliation":[{"name":"Department of Medicine SUNY Upstate Medical University  Syracuse New York USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-7835-1467","authenticated-orcid":false,"given":"Jean M.","family":"Sanger","sequence":"additional","affiliation":[{"name":"Department of Cell and Developmental Biology SUNY Upstate Medical University  Syracuse New York USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2281-8490","authenticated-orcid":false,"given":"Joseph W.","family":"Sanger","sequence":"additional","affiliation":[{"name":"Department of Cell and Developmental Biology SUNY Upstate Medical University  Syracuse New York USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,8,4]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yexcr.2008.09.001"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2222005120"},{"key":"e_1_2_12_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-019517720-6.50016-X"},{"key":"e_1_2_12_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/cm.70063"},{"key":"e_1_2_12_6_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.94.17.9493"},{"key":"e_1_2_12_7_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.35.2.405"},{"key":"e_1_2_12_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00109\u2010005\u20100033\u20101"},{"key":"e_1_2_12_9_1","doi-asserted-by":"publisher","DOI":"10.1023\/a:1005533013926"},{"key":"e_1_2_12_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0006\u20103495(98)77976\u20107"},{"key":"e_1_2_12_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/2021067b0"},{"key":"e_1_2_12_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/173973a0"},{"key":"e_1_2_12_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/173971a0"},{"key":"e_1_2_12_14_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.aan1133"},{"key":"e_1_2_12_15_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M114.550418"},{"key":"e_1_2_12_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097\u20100169(1997)37:3<183::AID\u2010CM1>3.0.CO;2\u20108"},{"key":"e_1_2_12_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/s0962\u20108924(03)00035\u20107"},{"key":"e_1_2_12_18_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M110.156646"},{"key":"e_1_2_12_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/cm.970280102"},{"key":"e_1_2_12_20_1","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/ddg020"},{"key":"e_1_2_12_21_1","doi-asserted-by":"publisher","DOI":"10.1126\/scisignal.132pe37"},{"key":"e_1_2_12_22_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.98.3.825"},{"key":"e_1_2_12_23_1","first-page":"45","volume-title":"Myology","author":"Sanger J. W.","year":"2004"},{"key":"e_1_2_12_24_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10974\u2010005\u20109016\u20107"},{"key":"e_1_2_12_25_1","first-page":"39","volume-title":"Handb Exp Pharmacol","author":"Sanger J. W.","year":"2017"},{"key":"e_1_2_12_26_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200210140"},{"key":"e_1_2_12_27_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.201010099"},{"key":"e_1_2_12_28_1","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.abg7653"},{"key":"e_1_2_12_29_1","doi-asserted-by":"publisher","DOI":"10.1002\/cm.20265"},{"key":"e_1_2_12_30_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms22063058"},{"key":"e_1_2_12_31_1","doi-asserted-by":"publisher","DOI":"10.1002\/cm.20063"},{"key":"e_1_2_12_32_1","doi-asserted-by":"publisher","DOI":"10.1002\/cm.20542"},{"key":"e_1_2_12_33_1","doi-asserted-by":"publisher","DOI":"10.1002\/cm.21454"},{"key":"e_1_2_12_34_1","doi-asserted-by":"publisher","DOI":"10.1002\/cphy.c170023"},{"key":"e_1_2_12_35_1","doi-asserted-by":"publisher","DOI":"10.1002\/cm.21729"},{"key":"e_1_2_12_36_1","doi-asserted-by":"publisher","DOI":"10.1002\/cm.21725"},{"key":"e_1_2_12_37_1","doi-asserted-by":"publisher","DOI":"10.1002\/cm.27192"},{"key":"e_1_2_12_38_1","doi-asserted-by":"publisher","DOI":"10.1002\/cm.21841"},{"key":"e_1_2_12_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/s0022\u20102828(95)91731\u20104"},{"key":"e_1_2_12_40_1","doi-asserted-by":"publisher","DOI":"10.1529\/biophysj.103.022087"}],"container-title":["Cytoskeleton"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cm.70169","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/cm.70169","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cm.70169","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,4]],"date-time":"2026-08-04T08:21:11Z","timestamp":1785831671000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/cm.70169"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8,4]]},"references-count":39,"alternative-id":["10.1002\/cm.70169"],"URL":"https:\/\/doi.org\/10.1002\/cm.70169","archive":["Portico"],"relation":{},"ISSN":["1949-3584","1949-3592"],"issn-type":[{"value":"1949-3584","type":"print"},{"value":"1949-3592","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,8,4]]},"assertion":[{"value":"2026-02-20","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-06-25","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-08-04","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70169"}}