{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T12:15:40Z","timestamp":1789992940430,"version":"4.0.1"},"reference-count":36,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T00:00:00Z","timestamp":1789948800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T00:00:00Z","timestamp":1789948800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100000268","name":"Biotechnology and Biological Sciences Research Council","doi-asserted-by":"publisher","award":["BB\/Y01264X\/1"],"award-info":[{"award-number":["BB\/Y01264X\/1"]}],"id":[{"id":"10.13039\/501100000268","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000268","name":"Biotechnology and Biological Sciences Research Council","doi-asserted-by":"publisher","award":["BB\/S003789\/1"],"award-info":[{"award-number":["BB\/S003789\/1"]}],"id":[{"id":"10.13039\/501100000268","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100010269","name":"Wellcome Trust","doi-asserted-by":"publisher","award":["WT101885MA"],"award-info":[{"award-number":["WT101885MA"]}],"id":[{"id":"10.13039\/100010269","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000854","name":"Human Frontier Science Program","doi-asserted-by":"publisher","award":["RGP001\/2023"],"award-info":[{"award-number":["RGP001\/2023"]}],"id":[{"id":"10.13039\/501100000854","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cytoskeleton"],"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Actin filaments exhibit isoform and species\u2010specific mechanical properties that are commonly quantified via their persistence length, yet the reliability of such measurements depends not only on the analysis method but also on dataset characteristics. Here, we measure and compare the persistence lengths of actin filaments from\n                    <jats:italic>Schizosaccharomyces pombe<\/jats:italic>\n                    and\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Saccharomyces cerevisiae<\/jats:italic>\n                    <\/jats:styled-content>\n                    as two exemplars, focusing on statistical and methodological factors that influence their determination. We evaluate common cosine\u2010correlation\u2010based analysis strategies and systematically assess the impact of filament length and dataset size on the accuracy of extracted persistence lengths. Because the bending stiffness of actin filaments can scale non\u2010linearly with filament numbers in bundles, even small errors in the persistence length can have disproportionately large impact on the estimation of mechanical behavior. We validate our findings through comparative analysis utilizing simulated thermal fluctuations of semi\u2010flexible polymers. Our results reinforce the importance of filament length and dataset size for the reliability of persistence length estimation, suggesting that their influence may have been underestimated. We introduce a weighting strategy that reduces the influence of error\u2010dominated correlation regimes and improves the robustness of the analysis. This work contributes a quantitative framework for designing experiments and analyzing actin filament mechanics, ensuring robust and reproducible persistence length measurements. Furthermore, applying this approach revealed a significant difference in the persistence lengths of\n                    <jats:italic>S. pombe<\/jats:italic>\n                    and\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>S. cerevisiae<\/jats:italic>\n                    <\/jats:styled-content>\n                    actin filaments.\n                  <\/jats:p>","DOI":"10.1002\/cm.70193","type":"journal-article","created":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T11:53:29Z","timestamp":1789991609000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Quantifying Actin Persistence Length in\n                    <i>S. pombe<\/i>\n                    and\n                    <i>S. cerevisiae<\/i>\n                    : The Impact of Data Size, Filament Length and Fitting Strategy"],"prefix":"10.1002","author":[{"ORCID":"https:\/\/orcid.org\/0009-0006-4779-1067","authenticated-orcid":false,"given":"Peter","family":"Nietmann","sequence":"first","affiliation":[{"name":"University of Warwick Warwick Medical School  Coventry UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4832-1642","authenticated-orcid":false,"given":"Andrejus","family":"Suchenko","sequence":"additional","affiliation":[{"name":"University of Warwick Warwick Medical School  Coventry UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0773-2963","authenticated-orcid":false,"given":"Andreas","family":"Janshoff","sequence":"additional","affiliation":[{"name":"Georg\u2010August Universitaet Institute for Physical Chemistry  Goettingen Germany"},{"name":"Max Planck Institute for Medical Research Max Planck School Matter to Life  Heidelberg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1292-8602","authenticated-orcid":false,"given":"Mohan K.","family":"Balasubramanian","sequence":"additional","affiliation":[{"name":"University of Warwick Warwick Medical School  Coventry UK"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,9,21]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/cm.21138"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2026.117473"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-3495(99)77207-3"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0503732102"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-020-20827-4"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmb.2009.11.001"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bpj.2010.07.009"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.120.4.923"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0094766"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi048021j"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.241406"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jpcb.6b02741"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.270.19.11437"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/347095a0"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.2976\/1.3054712"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/recl.19490681203"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1186\/1751-0473-9-16"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1103\/PhysRevLett.89.258101"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bpj.2011.05.049"},{"key":"e_1_2_10_21_1","unstructured":"Nietmann P.2026.\u201cMatlab Code.\u201dAccessed February 25 2026.https:\/\/github.com\/PeterNWarwick\/LP_analysis_for_micro_filaments. 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