{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T11:10:09Z","timestamp":1790334609215,"version":"4.1.0"},"reference-count":44,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T00:00:00Z","timestamp":1790294400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T00:00:00Z","timestamp":1790294400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100000038","name":"Natural Sciences and Engineering Research Council of Canada","doi-asserted-by":"publisher","award":["RGPIN\u201003945"],"award-info":[{"award-number":["RGPIN\u201003945"]}],"id":[{"id":"10.13039\/501100000038","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cytoskeleton"],"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Kinesins family members (KIFs) are encoded in mammals by approximately 45 genes, which are grouped into 14 families based on structural and functional features. As cells increased in complexity, so too did the demand for specialized kinesin\u2010mediated processes, including intracellular transport, cytoskeletal organization, and cell division. One mechanism driving this diversity is alternative splicing, which can generate multiple protein isoforms with distinct properties. Here, we highlight examples of KIF isoforms that differ in cargo binding and biophysical motor behavior, as well as their roles in cell cycle regulation, development, and cancer. Moreover, using the HUGO and UniProt databases, we select a few KIF isoforms and propose functional implications based on sequence alterations from that of the canonical transcript and protein. Given that isoform\u2010specific functional differences may have important consequences, we emphasize the need for future studies to investigate kinesin isoforms to better understand their roles in cellular processes and disease.<\/jats:p>","DOI":"10.1002\/cm.70208","type":"journal-article","created":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T10:43:01Z","timestamp":1790332981000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Hot and Splicey: Kinesin Isoforms Seek Attention"],"prefix":"10.1002","author":[{"given":"C.","family":"Belhadi","sequence":"first","affiliation":[{"name":"Department of Molecular Biology and Biochemistry Simon Fraser University  Burnaby Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6967-4917","authenticated-orcid":false,"given":"M. A.","family":"Silverman","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology and Biochemistry Simon Fraser University  Burnaby Canada"},{"name":"Centre for Cell Biology, Development and Disease Simon Fraser University  Burnaby Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,9,25]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.192674"},{"key":"e_1_2_7_3_1","doi-asserted-by":"crossref","DOI":"10.1038\/s41598-017-17192-6","article-title":"KIF1Bbeta Increases ROS to Mediate Apoptosis and Reinforces Its Protein Expression Through O2\u2212 in a Positive Feedback Mechanism in Neuroblastoma","volume":"7","author":"Angelina C.","year":"2017","journal-title":"Scientific Reports"},{"key":"e_1_2_7_4_1","doi-asserted-by":"crossref","first-page":"437","DOI":"10.1038\/nrm.2017.27","article-title":"Alternative Splicing as a Regulator of Development and Tissue Identity","volume":"18","author":"Baralle F. E.","year":"2017","journal-title":"Nature Reviews. Molecular Cell Biology"},{"key":"e_1_2_7_5_1","doi-asserted-by":"crossref","first-page":"273","DOI":"10.1083\/jcb.201408056","article-title":"A Novel Assay Reveals Preferential Binding Between Rabs, Kinesins, and Specific Endosomal Subpopulations","volume":"208","author":"Bentley M.","year":"2015","journal-title":"Journal of Cell Biology"},{"key":"e_1_2_7_6_1","doi-asserted-by":"crossref","DOI":"10.3390\/proteomes9030038","article-title":"Proteomes Are of Proteoforms: Embracing the Complexity","volume":"9","author":"Carbonara K.","year":"2021","journal-title":"Proteomes"},{"key":"e_1_2_7_7_1","doi-asserted-by":"crossref","first-page":"335","DOI":"10.1007\/s00018-012-1108-0","article-title":"KIF1Bbeta Transports Dendritically Localized mRNPs in Neurons and Is Recruited to Synapses in an Activity\u2010Dependent Manner","volume":"70","author":"Charalambous D. C.","year":"2013","journal-title":"Cellular and Molecular Life Sciences"},{"key":"e_1_2_7_8_1","doi-asserted-by":"crossref","DOI":"10.3390\/biom15060789","article-title":"Alternative Splicing in Tumorigenesis and Cancer Therapy","volume":"15","author":"Chen H.","year":"2025","journal-title":"Biomolecules"},{"key":"e_1_2_7_9_1","doi-asserted-by":"crossref","first-page":"617","DOI":"10.1007\/s003359901056","article-title":"The Major Brain Isoform of kif1b Lacks the Putative Mitochondria\u2010Binding Domain","volume":"10","author":"Conforti L.","year":"1999","journal-title":"Mammalian Genome"},{"key":"e_1_2_7_10_1","doi-asserted-by":"crossref","first-page":"10114","DOI":"10.1073\/pnas.88.22.10114","article-title":"Molecular Genetics of Kinesin Light Chains: Generation of Isoforms by Alternative Splicing","volume":"88","author":"Cyr J. L.","year":"1991","journal-title":"Proceedings of the National Academy of Sciences of the United States of America"},{"key":"e_1_2_7_11_1","doi-asserted-by":"crossref","first-page":"1375","DOI":"10.1016\/j.ajpath.2016.01.011","article-title":"A Unique Role for Endothelial Cell Kinesin Light Chain 1, Variant 1 in Leukocyte Transendothelial Migration","volume":"186","author":"Cyrus B. F.","year":"2016","journal-title":"American Journal of Pathology"},{"key":"e_1_2_7_12_1","doi-asserted-by":"crossref","first-page":"7014","DOI":"10.1523\/JNEUROSCI.0054-16.2016","article-title":"Kif1B Interacts With KBP to Promote Axon Elongation by Localizing a Microtubule Regulator to Growth Cones","volume":"36","author":"Drerup C. M.","year":"2016","journal-title":"Journal of Neuroscience"},{"key":"e_1_2_7_13_1","doi-asserted-by":"crossref","first-page":"2121","DOI":"10.1101\/gr.2596504","article-title":"The Status, Quality, and Expansion of the NIH Full\u2010Length cDNA Project: The Mammalian Gene Collection (MGC)","volume":"14","author":"Gerhard D. S.","year":"2004","journal-title":"Genome Research"},{"key":"e_1_2_7_14_1","doi-asserted-by":"crossref","first-page":"113","DOI":"10.1159\/000518923","article-title":"A Novel Variant of the KIF11 Gene, c.2922G>T, Is Associated With Microcephaly by Affecting RNA Splicing","volume":"44","author":"Guo Z.","year":"2022","journal-title":"Developmental Neuroscience"},{"issue":"11","key":"e_1_2_7_15_1","doi-asserted-by":"crossref","first-page":"2047","DOI":"10.1242\/jcs.113.11.2047","article-title":"An Isoform of Kinesin Light Chain Specific for the Golgi Complex","volume":"113","author":"Gyoeva F. K.","year":"2000","journal-title":"Journal of Cell Science"},{"key":"e_1_2_7_16_1","doi-asserted-by":"crossref","first-page":"16","DOI":"10.1016\/j.yexcr.2015.02.016","article-title":"Kinesin Superfamily Proteins (KIFs): Various Functions and Their Relevance for Important Phenomena in Life and Diseases","volume":"334","author":"Hirokawa N.","year":"2015","journal-title":"Experimental Cell Research"},{"key":"e_1_2_7_17_1","doi-asserted-by":"crossref","first-page":"449","DOI":"10.1016\/S0896-6273(00)00124-0","article-title":"Axonal Transport of Amyloid Precursor Protein Is Mediated by Direct Binding to the Kinesin Light Chain Subunit of Kinesin\u2010I","volume":"28","author":"Kamal A.","year":"2000","journal-title":"Neuron"},{"key":"e_1_2_7_18_1","doi-asserted-by":"crossref","first-page":"333","DOI":"10.1091\/mbc.9.2.333","article-title":"A Specific Light Chain of Kinesin Associates With Mitochondria in Cultured Cells","volume":"9","author":"Khodjakov A.","year":"1998","journal-title":"Molecular Biology of the Cell"},{"key":"e_1_2_7_19_1","doi-asserted-by":"crossref","first-page":"914","DOI":"10.1016\/j.cell.2011.04.028","article-title":"Centriolar Kinesin Kif24 Interacts With CP110 to Remodel Microtubules and Regulate Ciliogenesis","volume":"145","author":"Kobayashi T.","year":"2011","journal-title":"Cell"},{"key":"e_1_2_7_20_1","doi-asserted-by":"crossref","first-page":"4919","DOI":"10.1038\/s44318-025-00503-3","article-title":"FTO Promotes Weight Gain via Altering Kif1a Splicing and Axonal Vesicle Trafficking in AgRP Neurons","volume":"44","author":"Kohno D.","year":"2025","journal-title":"EMBO Journal"},{"key":"e_1_2_7_21_1","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1083\/jcb.200408113","article-title":"A Standardized Kinesin Nomenclature","volume":"167","author":"Lawrence C. 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M.","year":"2010","journal-title":"Gene"},{"key":"e_1_2_7_24_1","doi-asserted-by":"crossref","first-page":"4791","DOI":"10.1182\/blood-2012-10-461392","article-title":"Congenital Dyserythropoietic Anemia Type III (CDA III) is Caused by a Mutation in Kinesin Family Member, KIF23","volume":"121","author":"Liljeholm M.","year":"2013","journal-title":"Blood"},{"key":"e_1_2_7_25_1","doi-asserted-by":"crossref","DOI":"10.3390\/life12091441","article-title":"Comprehensive Transcriptome Analysis Reveals Sex\u2010Specific Alternative Splicing Events in Zebrafish Gonads","volume":"12","author":"Lin X.","year":"2022","journal-title":"Life"},{"key":"e_1_2_7_26_1","doi-asserted-by":"crossref","DOI":"10.3389\/fncel.2025.1527305","article-title":"Research Progress of Kinesin Family in Neurological Diseases","volume":"19","author":"Liu S.","year":"2025","journal-title":"Frontiers in Cellular Neuroscience"},{"key":"e_1_2_7_27_1","doi-asserted-by":"crossref","first-page":"535","DOI":"10.1016\/j.cell.2016.03.014","article-title":"On the Dependency of Cellular Protein Levels on mRNA Abundance","volume":"165","author":"Liu Y.","year":"2016","journal-title":"Cell"},{"key":"e_1_2_7_28_1","doi-asserted-by":"crossref","first-page":"e202201470","DOI":"10.26508\/lsa.202201470","article-title":"KIF24 Depletion Induces Clustering of Supernumerary Centrosomes in PDAC Cells","volume":"5","author":"Mashima Y.","year":"2022","journal-title":"Life Science Alliance"},{"key":"e_1_2_7_29_1","doi-asserted-by":"crossref","first-page":"140","DOI":"10.1111\/j.1600-0854.2003.00165.x","article-title":"A Novel Kinesin\u2010Like Protein, KIF1Bbeta3 Is Involved in the Movement of Lysosomes to the Cell Periphery in Non\u2010Neuronal Cells","volume":"5","author":"Matsushita M.","year":"2004","journal-title":"Traffic"},{"key":"e_1_2_7_30_1","doi-asserted-by":"crossref","first-page":"1647","DOI":"10.1111\/j.1600-0854.2009.00975.x","article-title":"Altered Motor Activity of Alternative Splice Variants of the Mammalian Kinesin\u20103 Protein KIF1B","volume":"10","author":"Matsushita M.","year":"2009","journal-title":"Traffic"},{"key":"e_1_2_7_31_1","doi-asserted-by":"crossref","first-page":"576","DOI":"10.1034\/j.1600-0854.2003.00113.x","article-title":"Alternatively Spliced Products of the Human Kinesin Light Chain 1 (KNS2) Gene","volume":"4","author":"McCart A. 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Identification and Characterization of the Encoded Proteins","volume":"273","author":"Rahman A.","year":"1998","journal-title":"Journal of Biological Chemistry"},{"key":"e_1_2_7_38_1","doi-asserted-by":"crossref","first-page":"884","DOI":"10.1101\/gad.1648608","article-title":"The Kinesin KIF1Bbeta Acts Downstream From EglN3 to Induce Apoptosis and Is a Potential 1p36 Tumor Suppressor","volume":"22","author":"Schlisio S.","year":"2008","journal-title":"Genes & Development"},{"key":"e_1_2_7_39_1","doi-asserted-by":"crossref","first-page":"11393","DOI":"10.1074\/jbc.M211874200","article-title":"Association of the Kinesin Motor KIF1A With the Multimodular Protein Liprin\u2010Alpha","volume":"278","author":"Shin H.","year":"2003","journal-title":"Journal of Biological Chemistry"},{"key":"e_1_2_7_40_1","doi-asserted-by":"crossref","DOI":"10.1186\/s12885-015-1987-1","article-title":"Evaluation of KIF23 Variant 1 Expression and Relevance as a Novel Prognostic Factor in Patients With Hepatocellular Carcinoma","volume":"15","author":"Sun X.","year":"2015","journal-title":"BMC Cancer"},{"key":"e_1_2_7_41_1","doi-asserted-by":"crossref","first-page":"233","DOI":"10.1186\/s12935-020-01323-7","article-title":"Kinesin Superfamily Protein 21B Acts as an Oncogene in Non\u2010Small Cell Lung Cancer","volume":"20","author":"Sun Z. G.","year":"2020","journal-title":"Cancer Cell International"},{"key":"e_1_2_7_42_1","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1016\/S0092-8674(85)80099-4","article-title":"Identification of a Novel Force\u2010Generating Protein, Kinesin, Involved in Microtubule\u2010Based Motility","volume":"42","author":"Vale R. D.","year":"1985","journal-title":"Cell"},{"key":"e_1_2_7_43_1","doi-asserted-by":"crossref","DOI":"10.1016\/j.bcmd.2020.102483","article-title":"Differential Tissue Specific Expression of Kif23 Alternative Transcripts in Mice With the Human Mutation Causing Congenital Dyserythropoietic Anemia Type III","volume":"85","author":"Vikberg A. L.","year":"2020","journal-title":"Blood Cells, Molecules & Diseases"},{"key":"e_1_2_7_44_1","doi-asserted-by":"crossref","DOI":"10.3389\/fonc.2021.708900","article-title":"Identification of Novel Molecular Therapeutic Targets and Their Potential Prognostic Biomarkers Among Kinesin Superfamily of Proteins in Pancreatic Ductal Adenocarcinoma","volume":"11","author":"Yang Y.","year":"2021","journal-title":"Frontiers in Oncology"},{"key":"e_1_2_7_45_1","doi-asserted-by":"crossref","first-page":"587","DOI":"10.1016\/S0092-8674(01)00363-4","article-title":"Charcot\u2010Marie\u2010Tooth Disease Type 2A Caused by Mutation in a Microtubule Motor KIF1Bbeta","volume":"105","author":"Zhao C.","year":"2001","journal-title":"Cell"}],"container-title":["Cytoskeleton"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cm.70208","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/cm.70208","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cm.70208","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T10:43:06Z","timestamp":1790332986000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/cm.70208"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,25]]},"references-count":44,"alternative-id":["10.1002\/cm.70208"],"URL":"https:\/\/doi.org\/10.1002\/cm.70208","archive":["Portico"],"relation":{},"ISSN":["1949-3584","1949-3592"],"issn-type":[{"value":"1949-3584","type":"print"},{"value":"1949-3592","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,25]]},"assertion":[{"value":"2026-07-03","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-09-21","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-09-25","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70208"}}