{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T04:49:40Z","timestamp":1792126180257,"version":"4.4.0"},"reference-count":53,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T00:00:00Z","timestamp":1792022400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T00:00:00Z","timestamp":1792022400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100009573","name":"Universidad Nacional de Rosario","doi-asserted-by":"publisher","award":["80020220600035UR"],"award-info":[{"award-number":["80020220600035UR"]}],"id":[{"id":"10.13039\/100009573","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100015890","name":"Agencia Santafesina de Ciencia, Tecnolog\u00eda e Innovaci\u00f3n","doi-asserted-by":"publisher","award":["PEICID 2023\u2010085"],"award-info":[{"award-number":["PEICID 2023\u2010085"]}],"id":[{"id":"10.13039\/501100015890","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cytoskeleton"],"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Microtubules play central roles in the organization and morphology of trypanosomatid parasites, forming highly specialized cytoskeletal structures such as the subpellicular corset, the flagellar axoneme, and the mitotic spindle. Functional specialization of MTs is proposed to be regulated by the \u201ctubulin code\u201d, which is defined by the combination of different \u03b1\u2010 and \u03b2\u2010tubulin isotypes, a set of post\u2010translational modifications and specific MT\u2010binding proteins. Although multiple tubulin post\u2010translational modifications have been described in trypanosomatids using specific antibodies or mass spectrometry, to date no comprehensive mapping has been reported in\n                    <jats:italic>Trypanosoma cruzi<\/jats:italic>\n                    , the causative agent of Chagas Disease. In the present work, we performed a high\u2010resolution proteomic analysis of PTMs present in \u03b1\u2010 and \u03b2\u2010tubulin subunits of the\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>T. cruzi<\/jats:italic>\n                    <\/jats:styled-content>\n                    Dm28c strain, using tubulin\u2010enriched extracts obtained by in\u00a0vitro polymerization. Multiple high\u2010confidence PTMs were identified by LC\u2013MS\/MS, including acetylation, methylation, phosphorylation, and polyglutamylation, for which many modified amino acids had not been previously reported in trypanosomatids. Structural mapping of these modifications onto a predicted \u03b1\/\u03b2\u2010tubulin heterodimer showed that most modified residues are located in solvent\u2010exposed regions of the protein. Together, these findings provide the first high\u2010confidence proteomic landscape of tubulin PTMs in\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>T. cruzi<\/jats:italic>\n                    <\/jats:styled-content>\n                    and support the existence of a complex tubulin code contributing to microtubule regulation in this parasite.\n                  <\/jats:p>","DOI":"10.1002\/cm.70211","type":"journal-article","created":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T04:40:56Z","timestamp":1792125656000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Proteomic Mapping of Novel Tubulin Post\u2010Translational Modifications in\n                    <i>Trypanosoma cruzi<\/i>\n                    Cytoskeleton"],"prefix":"10.1002","author":[{"given":"Gonzalo","family":"Martinez Peralta","sequence":"first","affiliation":[{"name":"Laboratorio de Biolog\u00eda y Bioqu\u00edmica de Trypanosoma cruzi, Instituto de Biolog\u00eda Molecular y Celular de Rosario (IBR), Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas (CONICET)  Rosario Argentina"},{"name":"Facultad de Ciencias Bioqu\u00edmicas y Farma c\u00e9uticas Universidad Nacional de Rosario (UNR)  Rosario Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daiana","family":"Baldelomar","sequence":"additional","affiliation":[{"name":"Facultad de Ciencias Bioqu\u00edmicas y Farma c\u00e9uticas Universidad Nacional de Rosario (UNR)  Rosario Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lucila","family":"Baldasseroni","sequence":"additional","affiliation":[{"name":"Facultad de Ciencias Bioqu\u00edmicas y Farma c\u00e9uticas Universidad Nacional de Rosario (UNR)  Rosario Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9285-385X","authenticated-orcid":false,"given":"Maria Azul","family":"de Hernandez","sequence":"additional","affiliation":[{"name":"Laboratorio de Biolog\u00eda y Bioqu\u00edmica de Trypanosoma cruzi, Instituto de Biolog\u00eda Molecular y Celular de Rosario (IBR), Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas (CONICET)  Rosario Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5986-7459","authenticated-orcid":false,"given":"Esteban Carlos","family":"Serra","sequence":"additional","affiliation":[{"name":"Laboratorio de Biolog\u00eda y Bioqu\u00edmica de Trypanosoma cruzi, Instituto de Biolog\u00eda Molecular y Celular de Rosario (IBR), Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas (CONICET)  Rosario Argentina"},{"name":"Facultad de Ciencias Bioqu\u00edmicas y Farma c\u00e9uticas Universidad Nacional de Rosario (UNR)  Rosario Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9518-8576","authenticated-orcid":false,"given":"Victoria Luc\u00eda","family":"Alonso","sequence":"additional","affiliation":[{"name":"Laboratorio de Biolog\u00eda y Bioqu\u00edmica de Trypanosoma cruzi, Instituto de Biolog\u00eda Molecular y Celular de Rosario (IBR), Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas (CONICET)  Rosario Argentina"},{"name":"Facultad de Ciencias Bioqu\u00edmicas y Farma c\u00e9uticas Universidad Nacional de Rosario (UNR)  Rosario Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,10,15]]},"reference":[{"issue":"8016","key":"e_1_2_12_2_1","doi-asserted-by":"crossref","first-page":"493","DOI":"10.1038\/s41586-024-07487-w","article-title":"Accurate Structure Prediction of Biomolecular Interactions with AlphaFold 3","volume":"630","author":"Abramson J.","year":"2024","journal-title":"Nature"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/S00436\u2010022\u201007619\u2010Z"},{"key":"e_1_2_12_4_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2021.642271"},{"key":"e_1_2_12_5_1","doi-asserted-by":"publisher","DOI":"10.1099\/mgen.0.000177"},{"key":"e_1_2_12_6_1","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.adv6637"},{"key":"e_1_2_12_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpara.2014.09.005"},{"key":"e_1_2_12_8_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.RA119.010951"},{"key":"e_1_2_12_9_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2415296122"},{"key":"e_1_2_12_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biochi.2017.08.005"},{"key":"e_1_2_12_11_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182018001828"},{"key":"e_1_2_12_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/J.EJOP.2021.125821"},{"key":"e_1_2_12_13_1","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1590\/S0074-02762009000900014","article-title":"Structural Organization of Trypanosoma cruzi","volume":"104","author":"De Souza W.","year":"2009","journal-title":"Mem\u00f3rias do Instituto Oswaldo Cruz"},{"key":"e_1_2_12_14_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.199471"},{"key":"e_1_2_12_15_1","doi-asserted-by":"crossref","first-page":"629","DOI":"10.1146\/annurev.micro.53.1.629","article-title":"The Cytoskeleton of Trypanosomatid Parasites","volume":"53","author":"Gull K.","year":"1999","journal-title":"Annual Review of Microbiology"},{"key":"e_1_2_12_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41580\u2010020\u20100214\u20103"},{"key":"e_1_2_12_17_1","doi-asserted-by":"publisher","DOI":"10.1242\/JCS.248047"},{"key":"e_1_2_12_18_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.261740"},{"key":"e_1_2_12_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbc.2021.100898"},{"key":"e_1_2_12_20_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro3274"},{"key":"e_1_2_12_21_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1011270"},{"key":"e_1_2_12_22_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00018\u2010015\u20102000\u20105"},{"key":"e_1_2_12_23_1","doi-asserted-by":"publisher","DOI":"10.1101\/pdb.prot5110"},{"key":"e_1_2_12_24_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.263431"},{"key":"e_1_2_12_25_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41594\u2010024\u201001406\u20103"},{"key":"e_1_2_12_26_1","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1042\/bj2650087","article-title":"Purification and Assembly In\u00a0Vitro of Tubulin From Trypanosoma brucei","volume":"265","author":"Macrae T. H.","year":"1990","journal-title":"Biochemical Journal"},{"key":"e_1_2_12_27_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0025381"},{"key":"e_1_2_12_28_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0046767"},{"key":"e_1_2_12_29_1","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.200700697"},{"key":"e_1_2_12_30_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jproteome.7b00603"},{"key":"e_1_2_12_31_1","doi-asserted-by":"publisher","DOI":"10.1074\/mcp.M800556\u2010MCP200"},{"key":"e_1_2_12_32_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jproteome.5c00107"},{"key":"e_1_2_12_33_1","doi-asserted-by":"publisher","DOI":"10.3390\/proteomes9010015"},{"key":"e_1_2_12_34_1","doi-asserted-by":"publisher","DOI":"10.1080\/19420862.2016.1228505"},{"key":"e_1_2_12_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2016.07.005"},{"key":"e_1_2_12_36_1","doi-asserted-by":"publisher","DOI":"10.2174\/0929867328666211126145721"},{"key":"e_1_2_12_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cellsig.2010.10.014"},{"key":"e_1_2_12_38_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkae1011"},{"key":"e_1_2_12_39_1","doi-asserted-by":"publisher","DOI":"10.1002\/pro.3943"},{"key":"e_1_2_12_40_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0003725"},{"key":"e_1_2_12_41_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.110.4.431"},{"key":"e_1_2_12_42_1","doi-asserted-by":"crossref","first-page":"439","DOI":"10.1083\/jcb.104.3.439","article-title":"Distinct Localization and Cell Cycle Dependence of COOH Terminally Tyrosinated Alpha\u2010Tubulin in the Microtubules of Trypanosoma brucei","volume":"104","author":"Sherwin T.","year":"1987","journal-title":"Journal of Cell Biology"},{"key":"e_1_2_12_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pt.2019.07.008"},{"key":"e_1_2_12_44_1","doi-asserted-by":"publisher","DOI":"10.1101\/GR.275368.121\/\u2010\/DC1"},{"key":"e_1_2_12_45_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467\u2010025\u201060677\u20106"},{"key":"e_1_2_12_46_1","doi-asserted-by":"publisher","DOI":"10.1242\/bio.062270"},{"key":"e_1_2_12_47_1","doi-asserted-by":"publisher","DOI":"10.1021\/pr4002492"},{"key":"e_1_2_12_48_1","doi-asserted-by":"publisher","DOI":"10.1021\/pr400086y"},{"key":"e_1_2_12_49_1","doi-asserted-by":"publisher","DOI":"10.1016\/J.CELREP.2019.11.074"},{"key":"e_1_2_12_50_1","volume-title":"Cytoskeleton\u2010Structure, Dynamics, Function and Disease","author":"Vidal J. C.","year":"2017"},{"key":"e_1_2_12_51_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467\u2010021\u201024376\u20102"},{"key":"e_1_2_12_52_1","doi-asserted-by":"publisher","DOI":"10.1155\/2010\/840518"},{"key":"e_1_2_12_53_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.meegid.2011.12.009"},{"key":"e_1_2_12_54_1","doi-asserted-by":"publisher","DOI":"10.2174\/1381612826999201203213527"}],"container-title":["Cytoskeleton"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cm.70211","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/cm.70211","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cm.70211","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T04:41:07Z","timestamp":1792125667000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/cm.70211"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,10,15]]},"references-count":53,"alternative-id":["10.1002\/cm.70211"],"URL":"https:\/\/doi.org\/10.1002\/cm.70211","archive":["Portico"],"relation":{},"ISSN":["1949-3584","1949-3592"],"issn-type":[{"value":"1949-3584","type":"print"},{"value":"1949-3592","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,10,15]]},"assertion":[{"value":"2026-04-09","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-10-08","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-10-15","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70211"}}