{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,25]],"date-time":"2026-08-25T23:06:13Z","timestamp":1787699173827,"version":"build-2784847793"},"reference-count":37,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2005,2,4]],"date-time":"2005-02-04T00:00:00Z","timestamp":1107475200000},"content-version":"vor","delay-in-days":6974,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cell Motil. Cytoskeleton"],"published-print":{"date-parts":[[1986,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Spreading and fully spread chick embryo fibroblasts (CEF) were examined by double\u2010label fluorescence microscopy using the actin\u2010specific probe rhodamine\u2010phalloidin and an antibody directed against CEF intermediate filaments (IF). During midspreading, a striking relationship became discernible: statistical analysis showed that approximately half of the cell population exhibited one or more phase\u2010dense, phalloidin\u2010binding nodules that appeared to act as foci from which IF diverged. Coincidence between actin\u2010containing structures and IF was not limited to these centers; IF could also frequently be seen running in close parallel arrays with stress fibers.<\/jats:p><jats:p>Ultrastructural analysis confirmed the presence of non\u2010membrane\u2010bound out\u2010pocketings along the length of stress fibers from which 10\u2010nm IF diverged. These structures varied in size and shape, and displayed a dense, fine fibrillar appearance. IF and microfilaments (MF) were distinguished by size and by decoration of MF with myosin subfragment\u20101. Other IF\u2010MF interactions were seen in cells of all stages: IF were observed to loop through stress fibers, most frequently at the cell margins. In colchicine\u2010treated cells, IF became redistributed into cables that often ran parallel and appeared to merge with stress fibers. Cytochalasin D\u2010treated CEF exhibited loose aggregates of actin\u2010containing material that appeared to be associated with IF.<\/jats:p><jats:p>These results suggest the possibility of an interaction between actin\u2010containing structures and IF, particularly during cell spreading in cultured fibroblasts.<\/jats:p>","DOI":"10.1002\/cm.970060406","type":"journal-article","created":{"date-parts":[[2005,2,24]],"date-time":"2005-02-24T04:31:13Z","timestamp":1109219473000},"page":"406-418","source":"Crossref","is-referenced-by-count":45,"title":["Relationship between intermediate filaments and microfilaments in cultured fibroblasts: Evidence for common foci during cell spreading"],"prefix":"10.1002","volume":"6","author":[{"given":"Kathleen J.","family":"Green","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John C.","family":"Talian","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert D.","family":"Goldman","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2005,2,4]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.96.6.1523"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4827(80)90428-0"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4827(75)90261-X"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.81.4.1117"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.92.2.575"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/cm.970040303"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.51.3.752"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.80.3.759"},{"key":"e_1_2_1_10_1","first-page":"91","volume-title":"Microtubules and Microtubule Inhibitors","author":"Goldman R. 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