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In this study, an assay was developed to analyze the kinetics and biological requirements for the tipping response: isolated flagella from <jats:italic>mt<\/jats:italic><jats:sup>\u2212<\/jats:sup> gametes of <jats:italic>C. reinhardtii<\/jats:italic> were allowed to agglutinate to the immotile flagella of <jats:italic>pf\u201018 mt<\/jats:italic><jats:sup>+<\/jats:sup> gametes, and their migration to the tips was monitored by phase microscopy. The tipping process is shown to require both adhesion and elevated levels of cAMP. The cAMP may activate tipping motors directly. In addition, cAMP stimulates the recruitment of agglutinins to flagellar surface to replace those inactivated by adhesion. These results are compared with previous studies on the tipping of flagellar surface proteins cross\u2010linked by soluble ligands, and an integrated model is presented. \u00a9 1993 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/cm.970250207","type":"journal-article","created":{"date-parts":[[2005,2,24]],"date-time":"2005-02-24T02:23:33Z","timestamp":1109211813000},"page":"179-189","source":"Crossref","is-referenced-by-count":25,"title":["Tipping of flagellar agglutinins by gametes of <i>Chlamydomonas reinhardtii<\/i>"],"prefix":"10.1002","volume":"25","author":[{"given":"Ursula W.","family":"Goodenough","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,2,4]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"crossref","first-page":"4593","DOI":"10.1016\/S0021-9258(18)34766-5","article-title":"Sexual agglutinins from the Chlamydomonas flagellar membrane. 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