{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T10:58:34Z","timestamp":1791284314676,"version":"4.1.0"},"reference-count":75,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2011,9,8]],"date-time":"2011-09-08T00:00:00Z","timestamp":1315440000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["chemistry-europe.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["ChemMedChem"],"published-print":{"date-parts":[[2011,11,4]]},"DOI":"10.1002\/cmdc.201100346","type":"journal-article","created":{"date-parts":[[2011,9,8]],"date-time":"2011-09-08T10:25:57Z","timestamp":1315477557000},"page":"1961-1966","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":35,"title":["Inhibition of Aminoglycoside\u2010Deactivating Enzymes APH(3\u2032)\u2010IIIa and AAC(6\u2032)\u2010Ii by Amphiphilic Paromomycin O2\u2032\u2032\u2010Ether Analogues"],"prefix":"10.1002","volume":"6","author":[{"given":"Janek","family":"Szychowski","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiro","family":"Kondo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Omar","family":"Zahr","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karine","family":"Auclair","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eric","family":"Westhof","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephen","family":"Hanessian","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeffrey W.","family":"Keillor","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2011,9,8]]},"reference":[{"key":"e_1_2_4_1_2","doi-asserted-by":"publisher","DOI":"10.1128\/9781555817886"},{"key":"e_1_2_4_2_2","first-page":"16","volume-title":"Antimicrobial Agents: Antibacterials and Antifungals","year":"2005"},{"key":"e_1_2_4_3_2","unstructured":"For example see:"},{"key":"e_1_2_4_4_2","doi-asserted-by":"publisher","DOI":"10.7164\/antibiotics.24.485"},{"key":"e_1_2_4_5_2","doi-asserted-by":"publisher","DOI":"10.7164\/antibiotics.25.695"},{"key":"e_1_2_4_6_2","doi-asserted-by":"publisher","DOI":"10.7164\/antibiotics.26.705"},{"key":"e_1_2_4_7_2","doi-asserted-by":"publisher","DOI":"10.1139\/v78-242"},{"key":"e_1_2_4_8_2","doi-asserted-by":"publisher","DOI":"10.1038\/327389a0"},{"key":"e_1_2_4_9_2","doi-asserted-by":"publisher","DOI":"10.1006\/jmbi.1996.0526"},{"key":"e_1_2_4_10_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.274.5291.1367"},{"key":"e_1_2_4_11_2","unstructured":"\u00a0"},{"key":"e_1_2_4_12_2","first-page":"423","volume":"3","author":"Hofstadler S. A.","year":"2000","journal-title":"Curr. Opin. Drug Discovery Dev."},{"key":"e_1_2_4_13_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.96.18.10129"},{"key":"e_1_2_4_14_2","unstructured":"\u00a0"},{"key":"e_1_2_4_15_2","doi-asserted-by":"publisher","DOI":"10.1006\/jmbi.1997.1551"},{"key":"e_1_2_4_16_2","doi-asserted-by":"publisher","DOI":"10.1006\/jmbi.1997.1552"},{"key":"e_1_2_4_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0969-2126(02)00934-6"},{"key":"e_1_2_4_18_2","unstructured":"\u00a0"},{"key":"e_1_2_4_19_2","doi-asserted-by":"publisher","DOI":"10.1038\/35030019"},{"key":"e_1_2_4_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0969-2126(01)00629-3"},{"key":"e_1_2_4_21_2","unstructured":"\u00a0"},{"key":"e_1_2_4_22_2","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/17.24.7490"},{"key":"e_1_2_4_23_2","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/18.13.3800"},{"key":"e_1_2_4_24_2","doi-asserted-by":"publisher","DOI":"10.1038\/72364"},{"key":"e_1_2_4_25_2","unstructured":"\u00a0"},{"key":"e_1_2_4_26_2","doi-asserted-by":"publisher","DOI":"10.1021\/jm061200"},{"key":"e_1_2_4_27_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tet.2006.10.079"},{"key":"e_1_2_4_28_2","doi-asserted-by":"publisher","DOI":"10.1002\/cmdc.200700113"},{"key":"e_1_2_4_29_2","doi-asserted-by":"publisher","DOI":"10.1021\/jm0508437"},{"key":"e_1_2_4_30_2","doi-asserted-by":"publisher","DOI":"10.1002\/ange.200462092"},{"key":"e_1_2_4_30_3","doi-asserted-by":"publisher","DOI":"10.1002\/anie.200462092"},{"key":"e_1_2_4_31_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0040-4020(01)00194-6"},{"key":"e_1_2_4_32_2","unstructured":"\u00a0"},{"key":"e_1_2_4_33_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.carres.2006.09.014"},{"key":"e_1_2_4_34_2","doi-asserted-by":"publisher","DOI":"10.1002\/cbic.200600109"},{"key":"e_1_2_4_35_2","doi-asserted-by":"publisher","DOI":"10.1002\/ange.200351354"},{"key":"e_1_2_4_35_3","doi-asserted-by":"publisher","DOI":"10.1002\/anie.200351354"},{"key":"e_1_2_4_36_2","doi-asserted-by":"publisher","DOI":"10.1021\/ol026588r"},{"key":"e_1_2_4_37_2","doi-asserted-by":"publisher","DOI":"10.1021\/ja9910356"},{"key":"e_1_2_4_38_2","doi-asserted-by":"publisher","DOI":"10.1021\/ja972599h"},{"key":"e_1_2_4_39_2","unstructured":"\u00a0"},{"key":"e_1_2_4_40_2","doi-asserted-by":"publisher","DOI":"10.2165\/00003088-200342050-00007"},{"key":"e_1_2_4_41_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9343(02)01476-6"},{"key":"e_1_2_4_42_2","doi-asserted-by":"publisher","DOI":"10.1021\/cr030102i"},{"key":"e_1_2_4_43_2","doi-asserted-by":"publisher","DOI":"10.1021\/cr0301088"},{"key":"e_1_2_4_44_2","unstructured":"\u00a0"},{"key":"e_1_2_4_45_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)80274-3"},{"key":"e_1_2_4_46_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.270.42.24686"},{"key":"e_1_2_4_47_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi9603884"},{"key":"e_1_2_4_48_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi010504p"},{"key":"e_1_2_4_49_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi0256680"},{"key":"e_1_2_4_50_2","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/21.10.2323"},{"key":"e_1_2_4_51_2","unstructured":"\u00a0"},{"key":"e_1_2_4_52_2","doi-asserted-by":"crossref","first-page":"956","DOI":"10.1128\/AAC.41.5.956","volume":"41","author":"Wright G. D.","year":"1997","journal-title":"Antimicrob. Agents Chemother."},{"key":"e_1_2_4_53_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi035667n"},{"key":"e_1_2_4_54_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0969-2126(99)80066-5"},{"key":"e_1_2_4_55_2","doi-asserted-by":"publisher","DOI":"10.1110\/ps.0233503"},{"key":"e_1_2_4_56_2","unstructured":"\u00a0"},{"key":"e_1_2_4_57_2","doi-asserted-by":"publisher","DOI":"10.1038\/nsmb.1978"},{"key":"e_1_2_4_58_2","doi-asserted-by":"publisher","DOI":"10.1002\/chem.200802172"},{"key":"e_1_2_4_59_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bmcl.2008.09.004"},{"key":"e_1_2_4_60_2","doi-asserted-by":"publisher","DOI":"10.1021\/jm060732n"},{"key":"e_1_2_4_61_2","doi-asserted-by":"publisher","DOI":"10.1002\/ange.200501399"},{"key":"e_1_2_4_61_3","doi-asserted-by":"publisher","DOI":"10.1002\/anie.200501399"},{"key":"e_1_2_4_62_2","unstructured":"\u00a0"},{"key":"e_1_2_4_63_2","doi-asserted-by":"publisher","DOI":"10.1021\/jm800345u"},{"key":"e_1_2_4_64_2","doi-asserted-by":"publisher","DOI":"10.1021\/jm1000437"},{"key":"e_1_2_4_65_2","doi-asserted-by":"publisher","DOI":"10.1021\/ja0543144"},{"key":"e_1_2_4_66_2","doi-asserted-by":"publisher","DOI":"10.1021\/ja050918w"},{"key":"e_1_2_4_67_2","doi-asserted-by":"publisher","DOI":"10.1021\/ja993452o"},{"key":"e_1_2_4_68_2","doi-asserted-by":"publisher","DOI":"10.1021\/ja00106a009"},{"key":"e_1_2_4_69_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bmcl.2007.03.014"},{"key":"e_1_2_4_70_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bmcl.2010.09.084"},{"key":"e_1_2_4_71_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi00188a024"},{"key":"e_1_2_4_72_2","unstructured":"For amikacin theKmvalue of 245\u2005\u03bcMreported by McKay et al. in Reference\u2005[24] (we observedKm=150\u2005\u03bcM) is higher than for other commercially available aminoglycosides and facilitates the detection of the phosphorylation at the concentrations used in the assay. Also amikacin is only singly phosphorylated whereas aminoglycosides such as paromomycin are phosphorylated twice and their biphasic phosphorylation curves make analysis more complicated."}],"container-title":["ChemMedChem"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fcmdc.201100346","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/chemistry-europe.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cmdc.201100346","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,12]],"date-time":"2025-10-12T14:27:47Z","timestamp":1760279267000},"score":1,"resource":{"primary":{"URL":"https:\/\/chemistry-europe.onlinelibrary.wiley.com\/doi\/10.1002\/cmdc.201100346"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2011,9,8]]},"references-count":75,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2011,11,4]]}},"alternative-id":["10.1002\/cmdc.201100346"],"URL":"https:\/\/doi.org\/10.1002\/cmdc.201100346","archive":["Portico"],"relation":{},"ISSN":["1860-7179","1860-7187"],"issn-type":[{"value":"1860-7179","type":"print"},{"value":"1860-7187","type":"electronic"}],"subject":[],"published":{"date-parts":[[2011,9,8]]},"assertion":[{"value":"2011-07-14","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2011-09-08","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}