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Its low immunogenicity, however, limits its application in the development of anticancer vaccines. To solve this problem, several <jats:italic>N<\/jats:italic>\u2010acyl\u2010modified TF derivatives were synthesized and conjugated with carrier protein CRM197 (a mutated diphtheria toxoid cross\u2010reactive material). The immunological results in BALB\/c mice demonstrated that these modified TF antigen conjugates could stimulate the production of higher titers of IgG antibodies that cross\u2010reacted with native TF antigen. These glycoconjugates showed strong lymphocyte proliferative response, suggesting that they can induce cellular immunity. Furthermore, the elicited antisera reacted strongly with TF\u2010positive tumor cells (4T1). In particular, the <jats:italic>N<\/jats:italic>\u2010monofluoroacetyl\u2010modified TF conjugate <jats:bold>4<\/jats:bold>\u2010CRM197 showed the strongest complement\u2010dependent cytotoxicity effect against 4T1 cells, implying the potential of this glycoconjugate as an anticancer vaccine.<\/jats:p>","DOI":"10.1002\/cmdc.201600094","type":"journal-article","created":{"date-parts":[[2016,4,14]],"date-time":"2016-04-14T02:36:22Z","timestamp":1460601382000},"page":"1090-1096","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":22,"title":["Synthesis and Evaluation of Glycoconjugates Comprising <i>N<\/i>\u2010Acyl\u2010Modified Thomsen\u2013Friedenreich Antigens as Anticancer Vaccines"],"prefix":"10.1002","volume":"11","author":[{"given":"Shuang","family":"Sun","sequence":"first","affiliation":[{"name":"State Key Laboratory of Natural and Biomimetic Drugs School of Pharmaceutical Sciences Peking University  Xue Yuan Road No.\u200538 Beijing 100191 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiu\u2010Jing","family":"Zheng","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Natural and Biomimetic Drugs School of Pharmaceutical Sciences Peking University  Xue Yuan Road No.\u200538 Beijing 100191 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chang\u2010Xin","family":"Huo","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Natural and Biomimetic Drugs School of Pharmaceutical Sciences Peking University  Xue Yuan Road No.\u200538 Beijing 100191 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chengcheng","family":"Song","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Natural and Biomimetic Drugs School of Pharmaceutical Sciences Peking University  Xue Yuan Road No.\u200538 Beijing 100191 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qin","family":"Li","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Natural and Biomimetic Drugs School of Pharmaceutical Sciences Peking University  Xue Yuan Road No.\u200538 Beijing 100191 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xin\u2010Shan","family":"Ye","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Natural and Biomimetic Drugs School of Pharmaceutical Sciences Peking University  Xue Yuan Road No.\u200538 Beijing 100191 China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2016,4,14]]},"reference":[{"key":"e_1_2_6_1_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.6729450"},{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4615-1267-7_24"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00005-009-0049-2"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02255855"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cbpa.2009.08.010"},{"key":"e_1_2_6_6_1","unstructured":"\u00a0"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1002\/eji.1830260307"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.180.2.739"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1074-7613(00)80006-0"},{"key":"e_1_2_6_10_1","unstructured":"\u00a0"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1521-3773(20000303)39:5<836::AID-ANIE836>3.0.CO;2-I"},{"key":"e_1_2_6_11_3","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1521-3757(20000303)112:5<882::AID-ANGE882>3.0.CO;2-1"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0968-0896(02)00135-9"},{"key":"e_1_2_6_13_1","doi-asserted-by":"crossref","first-page":"1708","DOI":"10.4049\/jimmunol.137.5.1708","volume":"137","author":"Jennings H. 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