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In the present work, the influence of hydrophobicity, fluorination, and distribution of cationic\/hydrophobic residues on antimicrobial, hemolytic, and cytotoxic properties of \u03b1\u2010peptide\/\u03b2\u2010peptoid hybrids were investigated. An array of 22 peptidomimetics was tested. Analogues with enhanced hydrophobicity were found to exhibit increased activity against Gram\u2010positive bacteria. Incorporation of fluorinated residues into the peptidomimetics conferred increased potency against Gram\u2010positive bacteria, while hemolytic properties and activity against Gram\u2010negative bacteria depended on the degree and type of fluorination. Generally, shorter oligomers were less potent than the corresponding longer analogues. However, some short analogues exhibited equal or higher antimicrobial activity. The alternating hydrophobic\/cationic design proved superior to other distribution patterns of cationic side chains and hydrophobic moieties.<\/jats:p>","DOI":"10.1002\/cmdc.201600553","type":"journal-article","created":{"date-parts":[[2017,1,4]],"date-time":"2017-01-04T11:52:42Z","timestamp":1483530762000},"page":"312-318","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":53,"title":["Lysine\u2010Based \u03b1\u2010Peptide\/\u03b2\u2010Peptoid Peptidomimetics: Influence of Hydrophobicity, Fluorination, and Distribution of Cationic Charge on Antimicrobial Activity and Cytotoxicity"],"prefix":"10.1002","volume":"12","author":[{"given":"Natalia","family":"Molchanova","sequence":"first","affiliation":[{"name":"Department of Drug Design and Pharmacology Faculty of Health and Medical Sciences University of Copenhagen  Universitetsparken\u20052 2100 Copenhagen Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul R.","family":"Hansen","sequence":"additional","affiliation":[{"name":"Department of Drug Design and Pharmacology Faculty of Health and Medical Sciences University of Copenhagen  Universitetsparken\u20052 2100 Copenhagen Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter","family":"Damborg","sequence":"additional","affiliation":[{"name":"Department of Veterinary Disease Biology Faculty of Health and Medical Sciences University of Copenhagen  Stigb\u00f8jlen\u20054 1870 Frederiksberg\u2005C Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hanne M.","family":"Nielsen","sequence":"additional","affiliation":[{"name":"Department of Pharmacy Faculty of Health and Medical Sciences University of Copenhagen  Universitetsparken\u20052 2100 Copenhagen Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Henrik","family":"Franzyk","sequence":"additional","affiliation":[{"name":"Department of Drug Design and Pharmacology Faculty of Health and Medical Sciences University of Copenhagen  Universitetsparken\u20052 2100 Copenhagen Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2017,1,12]]},"reference":[{"key":"e_1_2_6_1_1","doi-asserted-by":"crossref","first-page":"22","DOI":"10.1186\/1476-0711-12-22","volume":"12","author":"Bassetti M.","year":"2013","journal-title":"Ann. 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