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In this study we exploit common structural features present in linezolid\u2010resistant forms of both methicillin\u2010resistant\n                    <jats:italic>Staphylococcus aureus<\/jats:italic>\n                    (MRSA) and vancomycin\u2010resistant\n                    <jats:italic>Enterococcus<\/jats:italic>\n                    (VRE) to redesign the antibiotic. Enabled by rapid and facile cryoEM structures, this process has identified (\n                    <jats:italic>S<\/jats:italic>\n                    )\u20102,2\u2010dichloro\u2010\n                    <jats:italic>N<\/jats:italic>\n                    \u2010((3\u2010(3\u2010fluoro\u20104\u2010morpholinophenyl)\u20102\u2010oxooxazolidin\u20105\u2010yl)methyl)acetamide (LZD\u2010\n                    <jats:bold>5<\/jats:bold>\n                    ) and (\n                    <jats:italic>S<\/jats:italic>\n                    )\u20102\u2010chloro\u2010\n                    <jats:italic>N<\/jats:italic>\n                    \u2010((3\u2010(3\u2010fluoro\u20104\u2010morpholinophenyl)\u20102\u2010oxooxazolidin\u20105\u2010yl)methyl) acetamide (LZD\u2010\n                    <jats:bold>6<\/jats:bold>\n                    ), which inhibit the ribosomal function and growth of linezolid\u2010resistant MRSA and VRE. The strategy discussed highlights the potential for cryoEM to facilitate the development of novel bioactive materials.\n                  <\/jats:p>","DOI":"10.1002\/cmdc.201900042","type":"journal-article","created":{"date-parts":[[2019,1,23]],"date-time":"2019-01-23T17:38:45Z","timestamp":1548265125000},"page":"527-531","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":32,"title":["cryoEM\u2010Guided Development of Antibiotics for Drug\u2010Resistant Bacteria"],"prefix":"10.1002","volume":"14","author":[{"given":"Matthew J.","family":"Belousoff","sequence":"first","affiliation":[{"name":"Infection and Immunity Program Department of Microbiology Monash University  Clayton 3800 Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hari","family":"Venugopal","sequence":"additional","affiliation":[{"name":"Ramaciotti Centre for Electron Microscopy Monash University  Clayton 3800 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