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All compounds exhibited potent inhibition of both isoforms, with <jats:italic>K<\/jats:italic><jats:sub>I<\/jats:sub> values of &lt;100\u2005nM against hCA\u2005IX. Compound <jats:bold>4\u2009b<\/jats:bold> was the best inhibitor (<jats:italic>K<\/jats:italic><jats:sub>I<\/jats:sub>=78.5\u2005nM). All the compounds inhibited hCA\u2005XIII in the low\u2010nanomolar to sub\u2010micromolar range, with compound <jats:bold>4\u2009b<\/jats:bold> again showing the best inhibition (<jats:italic>K<\/jats:italic><jats:sub>I<\/jats:sub>=76.3\u2005nM). With compound <jats:bold>4\u2009b<\/jats:bold> as a lead, more\u2010selective inhibitors of hCA\u2005IX and hCA\u2005XIII or dual hCA\u2005IX\/XIII inhibitors might be developed.<\/jats:p>","DOI":"10.1002\/cmdc.202000915","type":"journal-article","created":{"date-parts":[[2020,12,21]],"date-time":"2020-12-21T20:40:16Z","timestamp":1608583216000},"page":"1252-1256","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":23,"title":["Coumarin\u2010Thiourea Hybrids Show Potent Carbonic Anhydrase IX and XIII Inhibitory Action"],"prefix":"10.1002","volume":"16","author":[{"given":"Pavitra S.","family":"Thacker","sequence":"first","affiliation":[{"name":"Department of Medicinal Chemistry National Institute of Pharmaceutical Education and Research (NIPER)  Balanagar, Hyderabad Telangana State India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Danaboina","family":"Srikanth","sequence":"additional","affiliation":[{"name":"Department of Medicinal Chemistry National Institute of Pharmaceutical Education and Research (NIPER)  Balanagar, Hyderabad Telangana State India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrea","family":"Angeli","sequence":"additional","affiliation":[{"name":"Universit\u00e0 degli Studi di Firenze Neurofarba Dept. 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