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Although enzyme replacement therapy (ERT) is successfully applied using mannose\u2010exposed conjugated glucocerebrosidase, the lower stability of the enzyme in blood demands periodic intravenous administration that adds to the high cost of treatment. In this work, the enzyme \u03b2\u2010glucocerebrosidase was encapsulated inside virus\u2010like nanoparticles (VLPs) from brome mosaic virus (BMV), and their surface was functionalized with mannose groups for targeting to macrophages. The VLP nanoreactors showed significant GCase catalytic activity. Moreover, the Michaelis\u2013Menten constants for the free GCase enzyme (K<jats:sub>M<\/jats:sub>=0.29\u2005mM) and the functionalized nanoreactors (K<jats:sub>M<\/jats:sub>=0.32\u2005mM) were similar even after chemical modification. Importantly, the stability of enzymes under physiological conditions (pH\u20057.4, 37\u2009\u00b0C) was enhanced by \u224811\u2010fold after encapsulation; this is beneficial for obtaining a higher blood circulation half\u2010life, which may decrease the cost of therapy by reducing the requirement of multiple intravenous injections. Finally, the mannose receptor targeted enzymatic nanoreactors showed enhanced internalization into macrophage cells. Thus, the catalytic activity and cell targeting suggest the potential of these nanoreactors in ERT of Gaucher's disease.<\/jats:p>","DOI":"10.1002\/cmdc.202200384","type":"journal-article","created":{"date-parts":[[2022,8,2]],"date-time":"2022-08-02T22:05:40Z","timestamp":1659477940000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":24,"title":["Targeted Enzymatic VLP\u2010Nanoreactors with \u03b2\u2010Glucocerebrosidase Activity as Potential Enzyme Replacement Therapy for Gaucher's Disease"],"prefix":"10.1002","volume":"17","author":[{"given":"Kanchan","family":"Chauhan","sequence":"first","affiliation":[{"name":"Centro de Nanociencias y Nanotecnolog\u00eda Universidad Nacional Aut\u00f3noma de M\u00e9xico  Km 107 carretera Tijuana-Ensenada 22860 Ensenada Baja California Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cindy N.","family":"Olivares\u2010Medina","sequence":"additional","affiliation":[{"name":"Centro de Nanociencias y Nanotecnolog\u00eda Universidad Nacional Aut\u00f3noma de M\u00e9xico  Km 107 carretera Tijuana-Ensenada 22860 Ensenada Baja California Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria V.","family":"Villagrana\u2010Escare\u00f1o","sequence":"additional","affiliation":[{"name":"Centro de Nanociencias y Nanotecnolog\u00eda Universidad Nacional Aut\u00f3noma de M\u00e9xico  Km 107 carretera Tijuana-Ensenada 22860 Ensenada Baja California Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karla","family":"Ju\u00e1rez\u2010Moreno","sequence":"additional","affiliation":[{"name":"Centro de Nanociencias y Nanotecnolog\u00eda Universidad Nacional Aut\u00f3noma de M\u00e9xico  Km 107 carretera Tijuana-Ensenada 22860 Ensenada Baja California Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rub\u00e9n D.","family":"Cadena\u2010Nava","sequence":"additional","affiliation":[{"name":"Centro de Nanociencias y Nanotecnolog\u00eda Universidad Nacional Aut\u00f3noma de M\u00e9xico  Km 107 carretera Tijuana-Ensenada 22860 Ensenada Baja California Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana G.","family":"Rodr\u00edguez\u2010Hern\u00e1ndez","sequence":"additional","affiliation":[{"name":"Centro de Nanociencias y Nanotecnolog\u00eda Universidad Nacional Aut\u00f3noma de M\u00e9xico  Km 107 carretera Tijuana-Ensenada 22860 Ensenada Baja California Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1612-2996","authenticated-orcid":false,"given":"Rafael","family":"Vazquez\u2010Duhalt","sequence":"additional","affiliation":[{"name":"Centro de Nanociencias y Nanotecnolog\u00eda Universidad Nacional Aut\u00f3noma de M\u00e9xico  Km 107 carretera Tijuana-Ensenada 22860 Ensenada Baja California Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,8,23]]},"reference":[{"key":"e_1_2_9_1_1","doi-asserted-by":"publisher","DOI":"10.1002\/ajh.24491"},{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1177\/1060028013500469"},{"key":"e_1_2_9_3_1","first-page":"1","volume":"10","author":"Mistry P. 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