{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,25]],"date-time":"2025-10-25T12:49:41Z","timestamp":1761396581760,"version":"build-2065373602"},"reference-count":20,"publisher":"Wiley","issue":"18","license":[{"start":{"date-parts":[[2023,7,24]],"date-time":"2023-07-24T00:00:00Z","timestamp":1690156800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100009619","name":"Japan Agency for Medical Research and Development","doi-asserted-by":"publisher","award":["JP21fk0108094","21fk0108428"],"award-info":[{"award-number":["JP21fk0108094","21fk0108428"]}],"id":[{"id":"10.13039\/100009619","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["chemistry-europe.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["ChemMedChem"],"published-print":{"date-parts":[[2023,9,15]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Cryptococcosis has become a major health problem worldwide and caused morbidity and mortality in immunocompromised patients, especially those infected with human immunodeficiency virus (HIV). Despite the global distribution of cryptococcosis, the number and types of the available antifungals are limited, and the treatment outcomes in HIV patients are generally poor. In this study, we screened a compound library and identified one tetrazole derivative as an efficient inhibitor of <jats:italic>Cryptococcus neoformans<\/jats:italic> and <jats:italic>Cryptococcus gattii<\/jats:italic>. We further designed and synthesized a series of tetrazole derivatives and determined their structure\u2010activity relationship, demonstrating that tetrazole backbone\u2010containing compounds could be developed as novel antifungal drugs with distinct mechanisms against <jats:italic>Cryptococcus<\/jats:italic> spp. Our findings provide a starting point for novel target identification and structural optimization to develop a distinct class of therapeutics for patients with cryptococcosis.<\/jats:p>","DOI":"10.1002\/cmdc.202300157","type":"journal-article","created":{"date-parts":[[2023,7,10]],"date-time":"2023-07-10T07:05:56Z","timestamp":1688972756000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Screening and Synthesis of Tetrazole Derivatives that Inhibit the Growth of <i>Cryptococcus<\/i> Species"],"prefix":"10.1002","volume":"18","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2508-0882","authenticated-orcid":false,"given":"Nana","family":"Nakada","sequence":"first","affiliation":[{"name":"Health Center Nagasaki University  1-14 Bunkyo-machi Nagasaki 852-8521 Japan"},{"name":"Department of Respiratory Medicine Nagasaki University Hospital  1-7-1 Sakamoto Nagasaki 852-8523 Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0962-5758","authenticated-orcid":false,"given":"Taiga","family":"Miyazaki","sequence":"additional","affiliation":[{"name":"Department of Respiratory Medicine Nagasaki University Hospital  1-7-1 Sakamoto Nagasaki 852-8523 Japan"},{"name":"Faculty of Medicine University of Miyazaki  5200 Kihara, Kiyotake Miyazaki 889-1692 Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Satoshi","family":"Mizuta","sequence":"additional","affiliation":[{"name":"Center for Bioinformatics and Molecular Medicine Nagasaki University Graduate School of Biomedical Sciences  1-12-4 Sakamoto Nagasaki Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tatsuro","family":"Hirayama","sequence":"additional","affiliation":[{"name":"Department of Respiratory Medicine Nagasaki University Hospital  1-7-1 Sakamoto Nagasaki 852-8523 Japan"},{"name":"Department of Pharmacotherapeutics Nagasaki University Graduate School of Biomedical Sciences  1-7-1 Sakamoto Nagasaki 852-8501 Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Seiko","family":"Nakamichi","sequence":"additional","affiliation":[{"name":"Health Center Nagasaki University  1-14 Bunkyo-machi Nagasaki 852-8521 Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kohsuke","family":"Takeda","sequence":"additional","affiliation":[{"name":"Department of Cell Regulation Nagasaki University Graduate School of Biomedical Sciences  1-14 Bunkyo-machi Nagasaki 852-8521 Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hiroshi","family":"Mukae","sequence":"additional","affiliation":[{"name":"Department of Respiratory Medicine Nagasaki University Hospital  1-7-1 Sakamoto Nagasaki 852-8523 Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shigeru","family":"Kohno","sequence":"additional","affiliation":[{"name":"Department of Respiratory Medicine Nagasaki University Hospital  1-7-1 Sakamoto Nagasaki 852-8523 Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yoshimasa","family":"Tanaka","sequence":"additional","affiliation":[{"name":"Center for Medical Innovation Nagasaki University  1-7-1 Sakamoto Nagasaki Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2023,7,24]]},"reference":[{"key":"e_1_2_9_1_1","doi-asserted-by":"publisher","DOI":"10.1101\/cshperspect.a019760"},{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1093\/mmy\/myy086"},{"key":"e_1_2_9_3_1","unstructured":"\u00a0"},{"key":"e_1_2_9_4_2","doi-asserted-by":"publisher","DOI":"10.1086\/649858"},{"key":"e_1_2_9_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1474-4422(18)30030-9"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tim.2011.07.010"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(17)30243-8"},{"key":"e_1_2_9_8_1","first-page":"e01719","volume":"65","author":"Fernandes C. 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