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This study aimed to identify the inhibitory effect of salicin on <jats:italic>S. aureus<\/jats:italic>. Coagulase (Coa) activity was assessed using <jats:italic>in\u2005vitro<\/jats:italic> Coa assays and Western blot, thermal shift assay (TSA), fluorescence quenching and molecular docking experiments were conducted to verify the interaction between salicin and Coa. An <jats:italic>in\u2005vivo<\/jats:italic> mouse pneumonia model demonstrated that salicin can reduce the virulence of <jats:italic>S. aureus. In vitro<\/jats:italic> Coa assays elucidated that salicin directly inhibited Coa activity. The Western blot and TSA results suggested that salicin did not block the expression of Coa but affected the thermal stability of the protein by binding to Coa. The fluorescence quenching, molecular docking and molecular dynamics assays have found that the most promising binding site between salicin and Coa was GLN\u201097. The pneumonia model of mice infected with <jats:italic>S. aureus<\/jats:italic> revealed that salicin could not only reduce the content of lung bacteria in mice but also prolong their survival. Salicin was identified as a novel anti\u2010infective candidate compound with the potential to target Coa and inhibit its activity by binding to it, which would facilitate the development of roadmaps for future research.<\/jats:p>","DOI":"10.1002\/cmdc.202300302","type":"journal-article","created":{"date-parts":[[2023,9,27]],"date-time":"2023-09-27T10:16:15Z","timestamp":1695809775000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Inhibitory Effect of Salicin on <i>Staphylococcus aureus<\/i> Coagulase"],"prefix":"10.1002","volume":"18","author":[{"given":"Yijing","family":"Jiang","sequence":"first","affiliation":[{"name":"Changchun University of Chinese Medicine  No.1035 Boshuo Road Jingyue National High-tech Industrial Development Zone Changchun 130117 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juan","family":"Hou","sequence":"additional","affiliation":[{"name":"Changchun University of Chinese Medicine  No.1035 Boshuo Road Jingyue National High-tech Industrial Development Zone Changchun 130117 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chang","family":"Liu","sequence":"additional","affiliation":[{"name":"Changchun University of Chinese Medicine  No.1035 Boshuo Road Jingyue National High-tech Industrial Development Zone Changchun 130117 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chunhui","family":"Zhao","sequence":"additional","affiliation":[{"name":"Changchun University of Chinese Medicine  No.1035 Boshuo Road Jingyue National High-tech Industrial Development Zone Changchun 130117 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yangming","family":"Xu","sequence":"additional","affiliation":[{"name":"Changchun University of Chinese Medicine  No.1035 Boshuo Road Jingyue National High-tech Industrial Development Zone Changchun 130117 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wu","family":"Song","sequence":"additional","affiliation":[{"name":"Changchun University of Chinese Medicine  No.1035 Boshuo Road Jingyue National High-tech Industrial Development Zone Changchun 130117 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-3434-1149","authenticated-orcid":false,"given":"Zunhua","family":"Shu","sequence":"additional","affiliation":[{"name":"The Third Affiliated Hospital to Changchun University of Chinese Medicine  No.1643, Jingyue Street Nanguan District Changchun 130118 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bingmei","family":"Wang","sequence":"additional","affiliation":[{"name":"Changchun University of Chinese Medicine  No.1035 Boshuo Road Jingyue National High-tech Industrial Development Zone Changchun 130117 China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,10,5]]},"reference":[{"key":"e_1_2_10_1_1","doi-asserted-by":"publisher","DOI":"10.1542\/pir.2017-0224"},{"key":"e_1_2_10_2_1","doi-asserted-by":"crossref","unstructured":"C.\u2005A. 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