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The conjugation of aptamers with fluorochromes or nanostructures has allowed the design of new identification strategies for <jats:italic>Pseudomonas aeruginosa<\/jats:italic> with detection limits of up to 1\u2005cell\u2009\u22c5\u2009mL<jats:sup>\u22121<\/jats:sup>, and the synergy of aptamers with antibiotics, antimicrobial peptides and nanostructures has exhibited promising therapeutic qualities. Some selected aptamers against this bacterium have shown intrinsic antimicrobial activity. However, these aptamers have been poorly evaluated in clinical isolates and have shown decreased interactions for CF isolates, demonstrating, in these cases, uncommon phenotypes resulting from the selective qualities of this disease as well as the great adaptive capacity of the pathogen. 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