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The primary objective was to evaluate the inhibitory capabilities of these compounds against four prominent isoforms: the cytosolic hCA I and II, as well as the tumor\u2010associated membrane\u2010bound hCA IX and XII. Impressively, the majority of the tested compounds exhibited significant inhibition activity against the tumor\u2010associated isoforms hCA IX and XII, with K<jats:sub>I<\/jats:sub> values ranging from 29.2 to 293.3\u2005nM. Notably, compound <jats:bold>DK\u20108<\/jats:bold> displayed particularly robust inhibitory activity against the tumor\u2010associated membrane\u2010bound isoforms, hCA IX and XII, yielding K<jats:sub>I<\/jats:sub> values of 32.5 and 29.2\u2005nM, respectively. Additionally, another derivative, <jats:bold>DK\u20109<\/jats:bold>, containing a primary sulfonamide, exhibited notable inhibition against hCA XII with a K<jats:sub>I<\/jats:sub> value of 36.4\u2005nM. This investigation aimed to explore the structure\u2010activity relationships within these compounds, shedding light on how various substitutions and structural components influence their inhibitory potential. As a result, these compounds present promising candidates for further exploration in medicinal and pharmacological research. Their ability to selectively inhibit specific isoforms, particularly those associated with hypoxic tumors, suggests their potential as foundational compounds for the development of novel therapeutic agents.<\/jats:p>","DOI":"10.1002\/cmdc.202300626","type":"journal-article","created":{"date-parts":[[2024,1,9]],"date-time":"2024-01-09T06:24:50Z","timestamp":1704781490000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Exploring the potency of diazo\u2010coumarin containing hybrid molecules: Selective inhibition of tumor\u2010associated carbonic anhydrase isoforms IX and XII"],"prefix":"10.1002","volume":"19","author":[{"given":"G\u00f6n\u00fcl","family":"Yapar","sequence":"first","affiliation":[{"name":"Department of Chemistry Faculty of Arts and Sciences Istanbul Technical University  Istanbul 34469 T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nebih","family":"Lolak","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Chemistry Faculty of Pharmacy Ad\u0131yaman University  02040 Ad\u0131yaman T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alessandro","family":"Bonardi","sequence":"additional","affiliation":[{"name":"Universit\u00e0 degli Studi di Firenze, NEUROFARBA Dept. 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