{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T03:14:20Z","timestamp":1792120460262,"version":"4.4.0"},"reference-count":63,"publisher":"Wiley","issue":"21","license":[{"start":{"date-parts":[[2025,9,8]],"date-time":"2025-09-08T00:00:00Z","timestamp":1757289600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["chemistry-europe.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["ChemMedChem"],"published-print":{"date-parts":[[2025,11,6]]},"abstract":"<jats:p>\n                    Antimicrobial peptides (AMPs) have emerged as promising candidates for combating drug\u2010resistant pathogens and certain cancer types. However, their therapeutic applications are often limited by undesired hemolytic activity, while many AMPs exhibit only moderate potency. Herein, the \"helical wheel rotation\" strategy as a simple, cost\u2010effective, and modular approach to optimize the pharmacological properties of amphipathic\n                    <jats:italic>\u03b1<\/jats:italic>\n                    \u2010helical AMPs without altering their amino acid composition is explored. Using\n                    <jats:bold>BP52<\/jats:bold>\n                    as a model peptide, six rotational variants (\n                    <jats:bold>BP52\u2010A1<\/jats:bold>\n                    to\n                    <jats:bold>BP52\u2010A6<\/jats:bold>\n                    ) and two sequence\u2010modified derivatives (\n                    <jats:bold>BP52\u2010B1<\/jats:bold>\n                    and\n                    <jats:bold>BP52\u2010B2<\/jats:bold>\n                    ) are developed to assess their antimicrobial, anticancer, and hemolytic properties. Several derivatives, especially\n                    <jats:bold>BP52\u2010A6<\/jats:bold>\n                    , exhibit enhanced antimicrobial activity and reduced hemolysis while maintaining or improving potency toward cancer cells. Importantly, all derivatives show substantially reduced hemolytic activity compared to\n                    <jats:bold>BP52<\/jats:bold>\n                    . These findings highlight the potential of helical wheel rotation as a valuable peptide engineering strategy to fine\u2010tune selectivity and multifunctionality of AMPs for therapeutic applications.\n                  <\/jats:p>","DOI":"10.1002\/cmdc.202500316","type":"journal-article","created":{"date-parts":[[2025,9,8]],"date-time":"2025-09-08T19:15:22Z","timestamp":1757358922000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Optimizing Amphipathic Antimicrobial Peptides via Helical Wheel Rotation"],"prefix":"10.1002","volume":"20","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1077-4507","authenticated-orcid":false,"given":"Hai","family":"Bui Thi Phuong","sequence":"first","affiliation":[{"name":"Faculty of Pharmacy PHENIKAA University  Hanoi 12116 Vietnam"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nguyen\u2010Thi","family":"Phuong","sequence":"additional","affiliation":[{"name":"NTT Hi\u2010Tech Institute Nguyen Tat Thanh University  HCMC 70000 Vietnam"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1283-8324","authenticated-orcid":false,"given":"Le Minh","family":"Bui","sequence":"additional","affiliation":[{"name":"NTT Hi\u2010Tech Institute Nguyen Tat Thanh University  HCMC 70000 Vietnam"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hue","family":"Pham Thi","sequence":"additional","affiliation":[{"name":"Bioresource Research Center PHENIKAA University  Hanoi 12116 Vietnam"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thi Thu Phuong","family":"Tran","sequence":"additional","affiliation":[{"name":"Department of Life Sciences University of Science and Technology of Hanoi (USTH) Vietnam Academy of Science and Technology (VAST)  18 Hoang Quoc Viet Cau Giay 10000 Hanoi Vietnam"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thang","family":"Nguyen Quoc","sequence":"additional","affiliation":[{"name":"Faculty of Pharmaceutical Chemistry and Technology Hanoi University of Pharmacy  Hanoi 10000 Vietnam"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8732-8230","authenticated-orcid":false,"given":"Hiep Tuan","family":"Tran","sequence":"additional","affiliation":[{"name":"Faculty of Pharmacy PHENIKAA University  Hanoi 12116 Vietnam"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Minh","family":"Nguyen Hong","sequence":"additional","affiliation":[{"name":"Bioresource Research Center PHENIKAA University  Hanoi 12116 Vietnam"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4815-006X","authenticated-orcid":false,"given":"Huy","family":"Luong Xuan","sequence":"additional","affiliation":[{"name":"Faculty of Pharmacy PHENIKAA University  Hanoi 12116 Vietnam"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,9,8]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0924-8579(03)00180-8"},{"key":"e_1_2_8_3_1","doi-asserted-by":"crossref","unstructured":"H. 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