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This study investigates the antiviral activity and structural properties of two antimicrobial peptides, pantinin\u20101 and pantinin\u20102, both derived from the venom of the scorpion <jats:italic>Pandinus imperator<\/jats:italic>, against caprine herpesvirus 1 (CpHV\u20101) and bovine herpesvirus 1 (BoHV\u20101). The results obtained from the plaque reduction assay and the quantitative real\u2010time polymerase chain reaction (PCR) indicate that synthetic pantinin\u2010mimetic peptides exhibited potent antiviral effects at concentrations ranging from 6\u201325 \u00b5M, impairing viral infectivity through direct virucidal action and inhibition of the viral entry and fusion with host cell. To characterize their structural behavior, nuclear magnetic resonance spectroscopy is performed in aqueous and membrane\u2010mimetic environments (trifluoroethanol (TFE)\/H<jats:sub>2<\/jats:sub>O). In aqueous solution, both peptides predominantly adopted random coil conformations, with pantinin\u20102 showing greater secondary structure propensity. In TFE\/H<jats:sub>2<\/jats:sub>O, both peptides transitioned to <jats:italic>\u03b1<\/jats:italic>\u2010helical structures, which are often associated with membrane interaction and antiviral activity. These findings demonstrate that pantinin\u20101 and pantinin\u20102 possess promising antiviral properties, supporting their potential development as therapeutic agents against herpesviruses and other animal viral infections.<\/jats:p>","DOI":"10.1002\/cmdc.202500333","type":"journal-article","created":{"date-parts":[[2025,9,14]],"date-time":"2025-09-14T13:27:37Z","timestamp":1757856457000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Pantinin\u2010Derived Peptides against Veterinary Herpesviruses: Activity and Structural Characterization"],"prefix":"10.1002","volume":"20","author":[{"given":"Rosa","family":"Giugliano","sequence":"first","affiliation":[{"name":"Department of Experimental Medicine University of Campania \"Luigi Vanvitelli\"  80138 Naples Italy"},{"name":"Department of Veterinary Medicine and Animal Production University of Naples Federico II  80137 Naples 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