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Interestingly, this process is not significantly facilitated by H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub>, indicating that this intracellular oxidant does not alter edAFs in the cellular environment. To identify alternative endogenous oxidants, NO is investigated as a potential candidate. Under aerobic conditions, NO is found to efficiently induce the oxidation and decomposition of edAFs. This transformation is mediated by an electrophilic nitrosation reaction, followed by nitroso\u2010oxime tautomerism and subsequent degradation of the ferrocenyl moiety with the release of ligand\u2010derived oxime <jats:bold>7<\/jats:bold> and iron ions. These findings suggest that NO may play a key role in the intracellular modification of edAFs, potentially contributing to their anticancer activity or their metabolism or both. Building on this mechanism, an effective probe is developed for detecting NO in living cells and identifying sites of inflammation in vivo. 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