{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,14]],"date-time":"2026-07-14T10:00:00Z","timestamp":1784023200031,"version":"3.55.0"},"reference-count":26,"publisher":"Wiley","issue":"23","license":[{"start":{"date-parts":[[2025,10,16]],"date-time":"2025-10-16T00:00:00Z","timestamp":1760572800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004895","name":"European Social Fund","doi-asserted-by":"publisher","award":["MIS 6004807"],"award-info":[{"award-number":["MIS 6004807"]}],"id":[{"id":"10.13039\/501100004895","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["chemistry-europe.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["ChemMedChem"],"published-print":{"date-parts":[[2025,11,28]]},"abstract":"<jats:p>\n                    The anticancer potential of silver(I) metallodrugs incorporating mefenamic acid and mitochondriotropic agents in 3D spheroid models of hormone\u2010dependent breast cancer cells (MCF\u20107) is investigated within this work. The compounds, previously evaluated in 2D cultures, demonstrated potent antiproliferative activity with IC\n                    <jats:sub>50<\/jats:sub>\n                    values ranging from 1.01 to 5.20\u2009\u03bcM, significantly outperforming cisplatin. Here, 3D spheroids are employed to simulate the tumor microenvironment, assessing drug efficacy through morphological changes and apoptosis rates. Compounds 1\u20134 exhibited dose\u2010 and time\u2010dependent reductions in spheroid compactness and sphericity, with compound 2 achieving complete loss of compactness (0%) and sphericity (2%) at 10\u2009\u03bcM after 48\u2009h. Apoptotic activity is also significantly higher in 3D spheroids treated with these compounds with respect to their control (25.8%\u221241.4%), in contrast to cisplatin (24.4%) and untreated cells (8.0%). The results validate the superior efficacy of silver(I) complexes in disrupting tumor integrity and inducing apoptosis. This study underscores the potential of 3D spheroid models for preclinical evaluation and highlights silver(I) metallodrugs as promising candidates for breast cancer therapy. Future research should focus on in\u2009vivo validation to further explore their therapeutic applications.\n                  <\/jats:p>","DOI":"10.1002\/cmdc.202500414","type":"journal-article","created":{"date-parts":[[2025,10,16]],"date-time":"2025-10-16T22:49:03Z","timestamp":1760654943000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Monitoring Apoptotic Activity of Silver(I) Metallodrugs Containing Mefenamic Acid and Mitochondriotropic Agents in Artificial Breast Tumor Tissues"],"prefix":"10.1002","volume":"20","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6727-2711","authenticated-orcid":false,"given":"Christina N.","family":"Banti","sequence":"first","affiliation":[{"name":"Biological Inorganic Chemistry laboratory Department of Chemistry University of Ioannina  45110 Ioannina Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9556-6266","authenticated-orcid":false,"given":"Sotiris K.","family":"Hadjikakou","sequence":"additional","affiliation":[{"name":"Biological Inorganic Chemistry laboratory Department of Chemistry University of Ioannina  45110 Ioannina Greece"},{"name":"Institute of Materials Science and Computing University Research Center of Ioannina (URCI)  45110 Ioannina 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