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Triple\u2010negative breast cancer (TNBC) is a biologically aggressive tumour, characterised by high rates of metastasis. Improved chemotherapeutic approaches against BC are therefore an urgent clinical need. In this study, complexes with two and three metal centres linked by the biogenic polyamines: putrescine (Pt\n                    <jats:sub>2<\/jats:sub>\n                    Put\n                    <jats:sub>2<\/jats:sub>\n                    (NH\n                    <jats:sub>3<\/jats:sub>\n                    )\n                    <jats:sub>4<\/jats:sub>\n                    and Pd\n                    <jats:sub>2<\/jats:sub>\n                    Put\n                    <jats:sub>2<\/jats:sub>\n                    ) and spermidine (Pt\n                    <jats:sub>3<\/jats:sub>\n                    Spd\n                    <jats:sub>2<\/jats:sub>\n                    and Pd\n                    <jats:sub>3<\/jats:sub>\n                    Spd\n                    <jats:sub>2<\/jats:sub>\n                    ), as well as cisplatin were tested against non\u2010malignant (MCF\u201012A), a TNBC (MDA\u2010MB\u2010468) and a non\u2010TNBC (MCF\u20107) human cell lines by 3\u2010(4,5\u2010dimethylthiazol\u20102\u2010yl)\u22122,5\u2010diphenyltetrazolium bromide assay. Fourier transform infrared and Raman microspectroscopies allow to probe biological samples with unmatched sub\u2010cellular spatial resolution. The results revealed that the polynuclear complexes showed activity for the TNBC cell line. While Pd\n                    <jats:sub>3<\/jats:sub>\n                    Spd\n                    <jats:sub>2<\/jats:sub>\n                    showed significant impact on DNA (B\u2010DNA to A\u2010 or Z\u2010DNA), Pt\n                    <jats:sub>2<\/jats:sub>\n                    Put\n                    <jats:sub>2<\/jats:sub>\n                    (NH\n                    <jats:sub>3<\/jats:sub>\n                    )\n                    <jats:sub>4<\/jats:sub>\n                    had a stronger effect on proteins (seen through Amide II vibrational mode). In addition, these complexes exhibited a greater impact on\n                    <jats:italic>\u03b4<\/jats:italic>\n                    <jats:italic>C<\/jats:italic>\n                    =C\u2013H from phospholipids and CH\n                    <jats:sub>2<\/jats:sub>\n                    deformations from lipids. For non\u2010TNBC, Pd\n                    <jats:sub>3<\/jats:sub>\n                    Spd\n                    <jats:sub>2<\/jats:sub>\n                    also displayed a strong contribution from Z\u2010DNA (\n                    <jats:italic>\u03bd<\/jats:italic>\n                    OPO\n                    <jats:sub>backbone<\/jats:sub>\n                    ), whereas Pt\n                    <jats:sub>2<\/jats:sub>\n                    Put\n                    <jats:sub>2<\/jats:sub>\n                    (NH\n                    <jats:sub>3<\/jats:sub>\n                    )\n                    <jats:sub>4<\/jats:sub>\n                    had a significant impact on Amide I and Amide III signals. This knowledge is expected to contribute for the rational design of improved anticancer drugs, with a higher efficiency coupled to lower toxicity.\n                  <\/jats:p>","DOI":"10.1002\/cmdc.202500814","type":"journal-article","created":{"date-parts":[[2026,5,9]],"date-time":"2026-05-09T05:06:08Z","timestamp":1778303168000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Triple\u2010Negative Breast Cancer Versus Non\u2010Triple\u2010Negative Breast Cancer: The Impact of Pd and Pt Complexes on Basal\u2010Like Triple\u2010Negative Breast Cancer"],"prefix":"10.1002","volume":"21","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4477-6794","authenticated-orcid":false,"given":"Clara B.","family":"Martins","sequence":"first","affiliation":[{"name":"Molecular Physical\u2010Chemistry, LAQV\/REQUIMTE, Department of Chemistry University of Coimbra  Coimbra Portugal"},{"name":"Department of Life Sciences Faculty of Science and Technology University of Coimbra  Coimbra Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria P. M.","family":"Marques","sequence":"additional","affiliation":[{"name":"Molecular Physical\u2010Chemistry, LAQV\/REQUIMTE, Department of Chemistry University of Coimbra  Coimbra Portugal"},{"name":"Department of Life Sciences Faculty of Science and Technology University of Coimbra  Coimbra Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lu\u00eds A. E.","family":"Batista de Carvalho","sequence":"additional","affiliation":[{"name":"Molecular Physical\u2010Chemistry, LAQV\/REQUIMTE, Department of Chemistry University of Coimbra  Coimbra Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1280-3321","authenticated-orcid":false,"given":"Ana L. 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