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Current antibody\u2010based antivenoms show only limited effectiveness against local tissue damage and require complex manufacturing and cold chain logistics. To overcome these limitations, a fluorescence\u2010based assay was established to enable the sensitive detection of snake venom metalloprotease (SVMP) and serine protease (SVSP) activities in crude viper venoms from\n                    <jats:italic>Crotalus atrox<\/jats:italic>\n                    ,\n                    <jats:italic>Bothrops jararaca,<\/jats:italic>\n                    and\n                    <jats:italic>Echis carinatus<\/jats:italic>\n                    . The assays showed species\u2010specific activity profiles with detection limits in the sub\u2010microgram per 45 and 100\u2009\u00b5L range for SVSP and SVMP, respectively. Notably, protease activity in bovine and human blood sera, with only a slight loss of sensitivity compared to isolated venom in buffer, can be measured. Inhibitory effects of small molecule protease inhibitors, batimastat, marimastat, ilomastat, nafamostat, and leupeptin, were determined, showing strong SVMP or SVSP inhibition, respectively. Based on this proof\u2010of\u2010concept, the combination of such activity\u2010based assays with selective small\u2010molecule inhibitors could open new opportunities for rapid venom detection in diagnosis and complementary therapy, particularly in resource\u2010poor settings.\n                  <\/jats:p>","DOI":"10.1002\/cmdc.202501099","type":"journal-article","created":{"date-parts":[[2026,3,31]],"date-time":"2026-03-31T14:56:16Z","timestamp":1774968976000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Snake Venom Protease Detection and Inhibition in Serum"],"prefix":"10.1002","volume":"21","author":[{"ORCID":"https:\/\/orcid.org\/0009-0001-1116-8293","authenticated-orcid":false,"given":"Mareike","family":"Riedel","sequence":"first","affiliation":[{"name":"Institute of Pharmaceutical and Biomedical Science Johannes Gutenberg University  Mainz 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