{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T22:26:04Z","timestamp":1792016764068,"version":"4.4.0"},"reference-count":45,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2026,5,22]],"date-time":"2026-05-22T00:00:00Z","timestamp":1779408000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,5,22]],"date-time":"2026-05-22T00:00:00Z","timestamp":1779408000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["chemistry-europe.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["ChemMedChem"],"published-print":{"date-parts":[[2026,5,27]]},"abstract":"<jats:p>\n                    Melanoma is an aggressive skin cancer characterized by rapid progression and frequent chemoresistance, which limits the success of current therapies. Saquayamycin B1 (SQ\u2010B1), an angucycline isolated from\n                    <jats:italic>Streptomyces<\/jats:italic>\n                    sp. I072, was investigated for its antimelanoma activity with emphasis on redox disruption. The compound was purified using M9 fermentation followed by semipreparative liquid chromatography, and its structure was confirmed through 1D\/2D nuclear magnetic resonance (NMR) and mass spectrometry. Cytotoxicity assays (MTT, 72\u2009h) in SK\u2010MEL\u20105, \u2212113, \u2212117, and \u2212134 cells revealed IC\n                    <jats:sub>50<\/jats:sub>\n                    values between 5.01 and 0.99\u2009\u03bcM. Pretreatment with N\u2010acetylcysteine (NAC) rescued cell viability at IC\n                    <jats:sub>50<\/jats:sub>\n                    and sub\u2010IC\n                    <jats:sub>50<\/jats:sub>\n                    concentrations, supporting a ROS\u2010dependent mechanism. SQ\u2010B1 (4\u2009\u03bcM, 24\u2009h) reduced the GSH\/GSSG ratio (3.49 \u2009\u00b1\u2009 0.70 vs. 12.35\u2009 \u00b1\u2009 0.27 in controls) and increased O\n                    <jats:sup>2\u2022\u2212<\/jats:sup>\n                    and NO\n                    <jats:sup>\u2022<\/jats:sup>\n                    levels, indicating marked oxidative imbalance. Molecular docking suggested strong binding of SQ\u2010B1 to glutathione transferase (GSTP1\u20101;\n                    <jats:italic>p<\/jats:italic>\n                    \u2248 0.86) and glutathione reductase (GSR;\n                    <jats:italic>p<\/jats:italic>\n                    \u2248 0.97), with weaker affinity for GPx4 (\n                    <jats:italic>p<\/jats:italic>\n                    \u2248 0.35). Molecular dynamics confirmed stable interactions between SQ\u2010B1 and GSR (RMSD plateau\u2009~10\u2009ns) with a total interaction energy of \u2212201.0\u2009kJ\/mol. These findings indicate that SQ\u2010B1 induces cytotoxicity through targeting of key redox regulators, highlighting its potential as a redox\u2010modulating candidate for chemoresistant melanoma.\n                  <\/jats:p>","DOI":"10.1002\/cmdc.70308","type":"journal-article","created":{"date-parts":[[2026,5,23]],"date-time":"2026-05-23T03:39:57Z","timestamp":1779507597000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Redox Disruption Induced by Saquayamycin B1 Promotes Cytotoxicity in Resistant Melanoma Cells"],"prefix":"10.1002","volume":"21","author":[{"given":"Geovana Guedes","family":"Silvestre","sequence":"first","affiliation":[{"name":"Postgraduate Program of Bioactives Natural and Synthetics Products PgPNSB Federal University of Para\u00edba  Jo\u00e3o Pessoa Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thalisson Amorim 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