{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,30]],"date-time":"2026-05-30T22:01:51Z","timestamp":1780178511490,"version":"3.54.0"},"reference-count":46,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2026,5,27]],"date-time":"2026-05-27T00:00:00Z","timestamp":1779840000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,5,27]],"date-time":"2026-05-27T00:00:00Z","timestamp":1779840000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100002322","name":"Brazilian Federal Agency for the Support and Evaluation of Graduate Education","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"Brazilian National Council for Research and Development","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["chemistry-europe.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["ChemMedChem"],"published-print":{"date-parts":[[2026,5,27]]},"abstract":"<jats:p>\n                    Antibiotic resistance is a critical challenge for global public health, stimulating the search for effective therapeutic alternatives. In this context, antimicrobial peptides (AMPs) stand out as promising candidates due to their structural diversity and multiple mechanisms of action. In this study, AMPs present in the floral nectar of\n                    <jats:italic>Nicotiana<\/jats:italic>\n                    sp. were evaluated for antibacterial activity against\n                    <jats:italic>Staphylococcus epidermidis<\/jats:italic>\n                    ,\n                    <jats:italic>S. aureus<\/jats:italic>\n                    ,\n                    <jats:italic>Klebsiella pneumoniae<\/jats:italic>\n                    , and\n                    <jats:italic>Pseudomonas aeruginosa<\/jats:italic>\n                    . Nicotianin\u2010I (NI) was identified as the most active natural peptide, although with limited efficacy. Therefore, it was selected as a model for rational engineering. From its sequence, two bioinspired analogs, NI\u2010A and NI\u2010B, were developed and showed significantly increased antibacterial activity in a strain\u2010dependent manner, as demonstrated by planktonic growth inhibition and cell viability assays. Mechanistic analyses indicated that NI\u2010A acts predominantly by destabilizing bacterial membranes, while NI\u2010B induces oxidative stress. Structural characterization revealed improved amphipathicity and environment\u2010dependent folding behavior. Hemolysis assays demonstrated that only NI\u2010B maintains low toxicity against human erythrocytes. Overall, these results highlight rational peptide engineering as a powerful strategy to transform low\u2010activity natural AMPs into optimized antimicrobial candidates with defined mechanisms and therapeutic potential.\n                  <\/jats:p>","DOI":"10.1002\/cmdc.70321","type":"journal-article","created":{"date-parts":[[2026,5,27]],"date-time":"2026-05-27T18:03:47Z","timestamp":1779905027000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Rational Engineering of a Floral Nectar Peptide Generates Enhanced Antimicrobial Analogs with Different Modes of Action"],"prefix":"10.1002","volume":"21","author":[{"given":"D\u00e1rio R. A. L.","family":"Mour\u00e3o","sequence":"first","affiliation":[{"name":"Department of Biochemistry and Molecular Biology Federal University of Cear\u00e1 (Pici Campus)  Fortaleza Cear\u00e1 Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jo\u00e3o M.","family":"Matos\u2010Neto","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology Federal University of Cear\u00e1 (Pici Campus)  Fortaleza Cear\u00e1 Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Queilane L. S.","family":"Chaves","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology Federal University of Cear\u00e1 (Pici Campus)  Fortaleza Cear\u00e1 Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mariana F.","family":"Oliveira","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology Federal University of Cear\u00e1 (Pici Campus)  Fortaleza Cear\u00e1 Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Viviane O.","family":"Silva","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology Federal University of Cear\u00e1 (Pici Campus)  Fortaleza Cear\u00e1 Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ariel F. N.","family":"Mesquita","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology Federal University of Cear\u00e1 (Pici Campus)  Fortaleza Cear\u00e1 Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ubirajara M.","family":"Paz\u2010Junior","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology Federal University of Cear\u00e1 (Pici Campus)  Fortaleza Cear\u00e1 Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexandre H.","family":"Sampaio","sequence":"additional","affiliation":[{"name":"Department of Fishing Engineering Federal University of Cear\u00e1 (Pici Campus)  Fortaleza Cear\u00e1 Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R\u00f4mulo F.","family":"Carneiro","sequence":"additional","affiliation":[{"name":"Department of Fishing Engineering Federal University of Cear\u00e1 (Pici Campus)  Fortaleza Cear\u00e1 Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M\u00e1rcio V.","family":"Ramos","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology Federal University of Cear\u00e1 (Pici Campus)  Fortaleza Cear\u00e1 Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2203-1575","authenticated-orcid":false,"given":"Cleverson D. 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