{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,22]],"date-time":"2026-06-22T02:47:45Z","timestamp":1782096465695,"version":"3.54.5"},"reference-count":30,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2026,6,5]],"date-time":"2026-06-05T00:00:00Z","timestamp":1780617600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,6,5]],"date-time":"2026-06-05T00:00:00Z","timestamp":1780617600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100005010","name":"Associazione Italiana per la Ricerca sul Cancro","doi-asserted-by":"publisher","award":["2021\u2010ID. 25899"],"award-info":[{"award-number":["2021\u2010ID. 25899"]}],"id":[{"id":"10.13039\/501100005010","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100007479","name":"Fondazione Cassa di Risparmio di Padova e Rovigo","doi-asserted-by":"publisher","award":["55777 2020.0162\u2010ARREST\u2010COV"],"award-info":[{"award-number":["55777 2020.0162\u2010ARREST\u2010COV"]}],"id":[{"id":"10.13039\/100007479","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["chemistry-europe.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["ChemMedChem"],"published-print":{"date-parts":[[2026,6,15]]},"abstract":"<jats:p>Emerging viral threats highlight the urgent need for antivirals targeting viral replication mechanisms. Based on previous research, we expanded a series of asymmetric benzene\u20101,4\u2010disulfonamides. As these proved to be synthetically challenging and generally more toxic than expected, we optimized a hit structure by omitting one sulfonamide group. The resulting compound combines micromolar antiviral efficacy with low toxicity and straightforward synthesis. Molecular docking and enzyme\u2010linked immunosorbent assay (ELISA) assays confirmed that the compound targets the NS5 RNA polymerase domain of dengue virus\u20102, blocking its interaction with NS3 and thereby inhibiting viral replication. A focused series of structural analogs further demonstrated the essential contribution of each individual part of the hit molecule.<\/jats:p>","DOI":"10.1002\/cmdc.70337","type":"journal-article","created":{"date-parts":[[2026,6,6]],"date-time":"2026-06-06T01:14:02Z","timestamp":1780708442000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Synthesis and Evaluation of Aromatic Sulfonamide and Disulfonamide Derivatives as Dengue Virus NS3\u2013NS5 Interaction Inhibitors"],"prefix":"10.1002","volume":"21","author":[{"given":"Jakub","family":"Jan\u00e1\u010d","sequence":"first","affiliation":[{"name":"Department of Organic Chemistry University of Chemistry and Technology Prague  Prague Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jan","family":"Drahor\u00e1d","sequence":"additional","affiliation":[{"name":"Department of Organic Chemistry University of Chemistry and Technology Prague  Prague Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jakub","family":"Harval\u00edk","sequence":"additional","affiliation":[{"name":"Department of Organic Chemistry University of Chemistry and Technology Prague  Prague Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tereza","family":"Hor\u00e1\u010dkov\u00e1","sequence":"additional","affiliation":[{"name":"Department of Organic Chemistry University of Chemistry and Technology Prague  Prague Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manfred","family":"Brinker","sequence":"additional","affiliation":[{"name":"Department of Organic Chemistry University of Chemistry and Technology Prague  Prague Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8900-861X","authenticated-orcid":false,"given":"Arianna","family":"Loregian","sequence":"additional","affiliation":[{"name":"Department of Molecular Medicine University of Padua  Padua Italy"},{"name":"Microbiology and Virology Unit Padua University Hospital  Padua Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrea","family":"Brancale","sequence":"additional","affiliation":[{"name":"Department of Organic Chemistry University of Chemistry and Technology Prague  Prague Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0654-8045","authenticated-orcid":false,"given":"Beatrice","family":"Mercorelli","sequence":"additional","affiliation":[{"name":"Department of Molecular Medicine University of Padua  Padua Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8264-7032","authenticated-orcid":false,"given":"Petra","family":"Cu\u0159\u00ednov\u00e1","sequence":"additional","affiliation":[{"name":"Department of Organic Chemistry University of Chemistry and Technology Prague  Prague Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,6,5]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1136\/bmjgh\u20102023\u2010014688"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tmaid.2025.102841"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.02023\u201024"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkaf514"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0319207"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1005737"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejmech.2016.10.007"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejmech.2017.10.064"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1126\/scitranslmed.abb2181"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/cr.2017.88"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.antiviral.2017.04.015"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.antiviral.2017.12.018"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2014.09.113"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.02349\u201020"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2013.09.078"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586\u2010021\u201003990\u20106"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2025.1663404"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsmedchemlett.9b00619"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jmedchem.5b00108"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.1128\/mbio.03097\u201022"},{"key":"e_1_2_11_22_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.2042\u20107158.1962.tb11123.x"},{"key":"e_1_2_11_23_1","doi-asserted-by":"publisher","DOI":"10.1002\/ange.201305369"},{"key":"e_1_2_11_24_1","doi-asserted-by":"publisher","DOI":"10.15227\/orgsyn.060.0121"},{"key":"e_1_2_11_25_1","doi-asserted-by":"publisher","DOI":"10.1021\/jo901924m"},{"key":"e_1_2_11_26_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.5b00941"},{"key":"e_1_2_11_27_1","volume-title":"Maestro","author":"Schr\u00f6dinger","year":"2019"},{"key":"e_1_2_11_28_1","volume-title":"Molecular Operating Environment (MOE)","author":"Chemical Computing Group ULC","year":"2024"},{"key":"e_1_2_11_29_1","doi-asserted-by":"publisher","DOI":"10.1039\/d4py00558a"},{"key":"e_1_2_11_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tet.2011.05.001"},{"key":"e_1_2_11_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bmcl.2021.128007"}],"container-title":["ChemMedChem"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/chemistry-europe.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cmdc.70337","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/chemistry-europe.onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/cmdc.70337","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/chemistry-europe.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cmdc.70337","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,6,22]],"date-time":"2026-06-22T02:11:24Z","timestamp":1782094284000},"score":1,"resource":{"primary":{"URL":"https:\/\/chemistry-europe.onlinelibrary.wiley.com\/doi\/10.1002\/cmdc.70337"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,6,5]]},"references-count":30,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2026,6,15]]}},"alternative-id":["10.1002\/cmdc.70337"],"URL":"https:\/\/doi.org\/10.1002\/cmdc.70337","archive":["Portico"],"relation":{},"ISSN":["1860-7179","1860-7187"],"issn-type":[{"value":"1860-7179","type":"print"},{"value":"1860-7187","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,6,5]]},"assertion":[{"value":"2026-02-18","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-05-18","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-06-05","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70337"}}