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However, their potential application as bioactive compounds against trypanosomatid parasites remains unexplored. In this work, we evaluated for the first time the activity of decavanadate (V\n                    <jats:sub>10<\/jats:sub>\n                    ) and monosubstituted V\n                    <jats:sub>9<\/jats:sub>\n                    M structures with M\u2009=\u2009Pt(IV), Mo(VI), and W(VI) on\n                    <jats:italic>Trypanosoma cruzi<\/jats:italic>\n                    . In some cases, activity increased by 1\u20132 orders of magnitude compared to monomeric vanadate (EC\n                    <jats:sub>50<\/jats:sub>\n                    monomeric vanadate V\n                    <jats:sub>1<\/jats:sub>\n                    \u2009&gt;\u20091000\u2009\u00b5M in epimastigotes and &gt;100\u2009\u00b5M in trypomastigotes, EC\n                    <jats:sub>50<\/jats:sub>\n                    V\n                    <jats:sub>10<\/jats:sub>\n                    in epimastigotes\u2009= 1.5\u2009\u00b5M, EC\n                    <jats:sub>50<\/jats:sub>\n                    V\n                    <jats:sub>9<\/jats:sub>\n                    Pt and V\n                    <jats:sub>9<\/jats:sub>\n                    Mo in trypomastigotes\u2009=\u20098.1\u2009\u00b5M). Significant differences in activity were observed between the trypomastigote and epimastigote forms of the parasite. Cytotoxicity, evaluated in VERO cells, remained within the same order of magnitude for both decavanadate\u2010type structures and monomeric vanadate.\n                    <jats:sup>51<\/jats:sup>\n                    V NMR measurements performed in culture media showed that substitution significantly affects cluster stability and speciation. Metal uptake studies did not show differences that could explain the observed biological activity, and transcriptomic assays identified no significant impact at the gene expression level. These results are consistent with the interpretation that the effects occur at the proteomic level.\n                  <\/jats:p>","DOI":"10.1002\/cmdc.70354","type":"journal-article","created":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T03:33:13Z","timestamp":1782876793000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Speciation Studies of Monosubstituted Pt, Mo, and W Decavanadates in Culture Media and In\u2009Vitro Activity Against\n                    <i>Trypanosoma cruzi<\/i>"],"prefix":"10.1002","volume":"21","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6307-2309","authenticated-orcid":false,"given":"Rodrigo","family":"Moreira","sequence":"first","affiliation":[{"name":"\u00c1rea Qu\u00edmica Inorg\u00e1nica, Departamento Estrella Campos Facultad de Qu\u00edmica Universidad de la Rep\u00fablica  Montevideo Uruguay"},{"name":"Programa de Posgrado de Facultad de Qu\u00edmica Facultad de Qu\u00edmica Universidad de la Rep\u00fablica  Montevideo Uruguay"},{"name":"PEDECIBA \u2013 Programa de Desarrollo de las Ciencias B\u00e1sicas Ministerio de Educaci\u00f3n y Cultura Universidad de la Rep\u00fablica  Montevideo Uruguay"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gonzalo","family":"Hern\u00e1ndez","sequence":"additional","affiliation":[{"name":"Laboratorio de RMN Departamento de Qu\u00edmica Org\u00e1nica Facultad de Qu\u00edmica Universidad de la Rep\u00fablica  Montevideo Uruguay"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jonathan","family":"Bastidas","sequence":"additional","affiliation":[{"name":"\u00c1rea Qu\u00edmica Inorg\u00e1nica, Departamento Estrella Campos Facultad de Qu\u00edmica Universidad de la Rep\u00fablica  Montevideo Uruguay"},{"name":"Instituto de Investigaciones Una Salud Universidad de la Rep\u00fablica  Montevideo Uruguay"},{"name":"Secci\u00f3n Gen\u00f3mica Funcional Facultad de Ciencias Universidad 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