{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,15]],"date-time":"2026-07-15T09:06:16Z","timestamp":1784106376959,"version":"3.55.0"},"reference-count":30,"publisher":"Wiley","issue":"13","license":[{"start":{"date-parts":[[2026,7,12]],"date-time":"2026-07-12T00:00:00Z","timestamp":1783814400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,7,12]],"date-time":"2026-07-12T00:00:00Z","timestamp":1783814400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["chemistry-europe.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["ChemMedChem"],"published-print":{"date-parts":[[2026,7,14]]},"abstract":"<jats:p>\n                    Human carbonic anhydrases (hCAs) are zinc\u2010bound enzymes that play a critical role in pH regulation and ion balance. Among them, isoforms hCA IX and XII are overexpressed in tumor hypoxia and are implicated in tumor progression. In this study, new indole\u2010benzenesulfonamide derivatives with hydrazide and amide linkers were synthesized and evaluated against hCA I, II, IX, and XII. Among them, compounds\n                    <jats:bold>5c<\/jats:bold>\n                    and\n                    <jats:bold>5o<\/jats:bold>\n                    showed potent inhibition against hCA IX with\n                    <jats:italic>K<\/jats:italic>\n                    <jats:sub>\n                      <jats:italic>i<\/jats:italic>\n                    <\/jats:sub>\n                    values of 22.4 and 22.7\u2009nM, respectively, while compound\n                    <jats:bold>5h<\/jats:bold>\n                    showed potent inhibition against hCA XII (\n                    <jats:italic>K<\/jats:italic>\n                    <jats:sub>\n                      <jats:italic>i<\/jats:italic>\n                    <\/jats:sub>\n                    \u2009=\u200922.6\u2009nM). Halogenation at C\n                    <jats:sub>5<\/jats:sub>\n                    \/C\n                    <jats:sub>6<\/jats:sub>\n                    and\n                    <jats:italic>N<\/jats:italic>\n                    \u2010substitution on the indole moiety played significant roles in improving the potency and selectivity toward hCA IX and XII. Molecular docking studies revealed that the most active compounds formed stable coordination with Zn\n                    <jats:sup>2+<\/jats:sup>\n                    , hydrogen bonding with Thr199, Thr200, and Gln92, and pi\u2013pi stacking with His residues. ADMET predictions indicated that the lead compounds possess favorable drug\u2010likeness and safety profiles. Together, these results identify\n                    <jats:bold>5c<\/jats:bold>\n                    ,\n                    <jats:bold>5o<\/jats:bold>\n                    ,\n                    <jats:bold>5f<\/jats:bold>\n                    , and\n                    <jats:bold>5h<\/jats:bold>\n                    as promising leads for further structural modifications to develop anticancer agents that selectively target hCA IX and XII isoforms.\n                  <\/jats:p>","DOI":"10.1002\/cmdc.70374","type":"journal-article","created":{"date-parts":[[2026,7,13]],"date-time":"2026-07-13T01:11:07Z","timestamp":1783905067000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Synthesis and Biological Evaluation of Indole\u2010Benzene Sulfonamides as Carbonic Anhydrase II, IX, and XII Inhibitors"],"prefix":"10.1002","volume":"21","author":[{"given":"Vadakattu","family":"Manasa","sequence":"first","affiliation":[{"name":"Department of Chemical Sciences National Institute of Pharmaceutical Education and Research (NIPER)  Hyderabad Telangana India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-9200-9923","authenticated-orcid":false,"given":"Sabesan","family":"Jayaprakash","sequence":"additional","affiliation":[{"name":"Department of Chemical Sciences National Institute of Pharmaceutical Education and Research (NIPER)  Hyderabad Telangana India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6874-9419","authenticated-orcid":false,"given":"Niccolo`","family":"Paoletti","sequence":"additional","affiliation":[{"name":"Neurofarba Department Sezione di Scienze Farmaceutiche e Nutraceutiche Universit\u00e0 Degli Studi di Firenze  Sesto Fiorentino Florence 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