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Here, we describe four novel fluorescent tetramic acid derivatives as potential LD markers for live imaging in cellular and whole\u2010organism systems, and designate the compound with the most favorable properties,\n                    <jats:bold>1a<\/jats:bold>\n                    , for further characterization. The probe\n                    <jats:bold>1a<\/jats:bold>\n                    was evaluated in multiple cell lines, where it enabled clear visualization of intracellular LDs and showed strong colocalization with established lipid markers such as Nile Red and BODIPY 493\/503. Photophysical characterization revealed photostability and signal\u2010to\u2010noise characteristics enabling fluorescence imaging applications. In addition, cytotoxicity assays indicated minimal acute and delayed effects at imaging concentrations. Importantly,\n                    <jats:bold>1a<\/jats:bold>\n                    was successfully applied in a zebrafish model, enabling visualization of lipid\u2010rich structures in a whole\u2010organism context. Together, these findings identify\n                    <jats:bold>1a<\/jats:bold>\n                    as a useful LD fluorescent marker for live imaging applications both in vitro and in vivo.\n                  <\/jats:p>","DOI":"10.1002\/cmdc.70418","type":"journal-article","created":{"date-parts":[[2026,8,13]],"date-time":"2026-08-13T03:52:34Z","timestamp":1786593154000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Tetramic Acid\u2010Derived Blue\u2010Emitting Fluorescent Probe for Selective Lipid Droplet Imaging in Live Cells and Zebrafish"],"prefix":"10.1002","volume":"21","author":[{"ORCID":"https:\/\/orcid.org\/0009-0007-7425-685X","authenticated-orcid":false,"given":"Marija","family":"Grozdani\u0107","sequence":"first","affiliation":[{"name":"Institute for Biological Research \u201cSini\u0161a Stankovi\u0107\u201d \u2013 National Institute of the Republic of Serbia University of Belgrade Belgrade 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